Rapid Determination of Cell Morphology in Eucalyptus Wood by Fourier Transform Raman Spectroscopy

Abstract

Analysis of cell morphology in native wood is a lengthy, multistep procedure, although it has a marked effect on product quality and the use of wood. The feasibility of using FT-Raman spectroscopy for rapid determination of cell length and cell wall ratio as cell morphology in native wood was examined with the use of wood meals of two Eucalyptus species, including samples of various ages and colors. The second-derivative transformation of Raman spectroscopic data and the partial least-squares regression revealed highly significant correlations between conventionally measured and Raman predicted values for cell length and cell wall ratio with correlation coefficient r > 0.9 and 0.8, respectively, in the calibration (for known samples, n = 55) and in the prediction (for unknown samples, n = 25).

Keywords

Determination Cell morphology Native wood Fourier transform Raman spectroscopy Multivariate data analysis

Get full access to this article

View all access options for this article.

References

Dinwoodie

J. M.

, Tappi 48, 440 (1965).

Zobel

B. J.

and Van Buijtenen

J. P

, Wood Variation (Springer-Verlag, Berlin, 1989), p. 7.

Bradbury

S. J.

, in Light Microscopy in Biology, Lacey

A. J.

, Ed. (IRL Press, Oxford, 1989), p. 187.

Evans

, Holzforschung 48, 168 (1994).

Michell

A. J.

, Appita 47, 29 (1994).

Ona

Sonoda

Ito

Shibata

Kato

and Ootake

, J. Wood Chem. Technol. 17, 399 (1997).

Ona

Sonoda

Ito

Shibata

Katayama

Kato

and Ootake

, J. Wood Chem. Technol. 18, 27 (1998).

Ona

Sonoda

Ito

Shibata

Kato

and Ootake

, J. Wood Chem. Technol. 18, 43 (1998).

Agarwal

U. P.

and Atalla

R. H

, in Surface Analysis of Paper, Conners

T. E.

, Ed. (CRC Press, Boca Raton, Florida, 1995), p. 152.

10.

Hallmark

V. M.

, in Fourier Transform Raman Spectroscopy: From Concept to Experiment, Chase

D. B.

and Rabolt

J. F

, Eds. (Academic Press, San Diego, California, 1994), p. 73.

11.

Ona

Sonoda

Ito

Shibata

Tamai

and Kojima

, Appita 49, 325 (1996).

12.

Huber

and Prütz

, Holz als Roh-. Werk. 1, 377 (1938).

13.

Baba

Ona

Takabe

Ito

and Ito

, Mokuzai Gakkaishi 42, 795 (1996).

14.

Ona

Sonoda

Shibata

and Fukazawa

, Tappi 78, 121 (1995).

15.

Ona

Sonoda

Ito

and Shibata

, Holzforschung 51, 396 (1997).

16.

Stewart

Wilson

H. M.

Hendra

P. J.

and Morrison

I. M

, J. Agric. Food Chem. 43, 2219 (1995).

17.

Edwards

H. G. M.

and Farwell

D. W

, Spectrochim. Acta 50A, 807 (1994).

18.

Koenig

J. L.

, J. Polym. Sci. Part D 6, 59 (1972).

19.

She

C. Y.

Dinh

N. D.

and Tu

A. T

, Biochim. Biophys. Acta 372, 345 (1974).

20.

Dollish

F. R.

Fateley

W. G.

and Bentley

F. F

, Characteristic Raman Frequencies of Organic Compounds (John Wiley and Sons, New York, 1974).

21.

Kenton

R. C.

and Rubinovitz

R. L

, Appl. Spectrosc. 44, 1377 (1990).

22.

Agarwal

U. P.

and Atalla

R. H

, Planta 169, 325 (1986).

23.

Agarwal

U. P.

and Ralph

S. A

, Appl. Spectrosc. 51, 1648 (1997).

24.

Wiley

J. H.

and Atalla

R. H

, Carbohyd. Res. 160, 113 (1987).

25.

Agarwal

U. P.

Atalla

R. H.

and Forsskåahl

, Holzforschung 49, 300 (1995).

26.

Atalla

R. H.

, J. Wood Chem. Technol. 7, 115 (1987).