A strategy for the infrared spectroscopic analysis of drug metabolites in high-pressure liquid chromatography (HPLC) effluent is presented with a primary focus on delivery of metabolite structure as opposed to development of dedicated or automated instrumentation. This paper stresses the complementary nature of on-line (solution phase) and off-line (condensed phase) HPLC Fourier transform infrared (FT-IR) implementations for structural elucidation of drug metabolites.
SocratesG., Infrared Characteristic Group Frequencies (John Wiley and Sons, New York1980).
13.
Lin-VeinD.ColthupN. B.FateleyW. G.GrasselliJ. G., The Handbook of Infrared and Raman Characteristic Frequencies of Organic Molecules (Academic Press, New York1991).
14.
SisenwineS. F.TioC. O.RueliusH. W., Pharmacoligist12, 272 (1970).
