Abstract
Dynamic FT-IR spectra in the amide I region were measured for silk fibroin cast films. Phase analyses of the dynamic spectra were carried out in order to separate them into several components which are due to the secondary structures of fibroin. These observations showed that peak positions of the amide I bands were in good agreement with those obtained by Fourier self-deconvolution procedures. In conclusion, phase analysis of dynamic FT-IR spectra is a powerful technique for separation of highly overlapping bands.
Keywords
Get full access to this article
View all access options for this article.
References
1.
Kauppinen
J. K.
Moffat
D. J.
Mantsch
H. H.
and
Cameron
D. G
, Appl. Spectrosc. 35 , 271 (1981 ).
2.
Susi
H.
and
Byler
D. M
, Biochem. Biophys. Res. Commun. 115 , 391 (1983 ).
3.
Byler
D. M.
and
Susi
H
, Biopolymers 25 , 469 (1986 ).
4.
Surewicz
W. K.
and
Mantsch
H. H
, Biochim. Biophys. Acta. 952 , 115 (1988 ).
5.
Jackson
M.
Haris
P. I.
and
Chapman
D
, J. Mol. Struct. 214 , 329 (1989 ).
6.
Surewicz
W. K.
Mantsch
H. H.
and
Chapman
D
, Biochemistry 32 , 389 (1993 ).
7.
Noda
I.
, J. Am. Chem. Soc. 111 , 8116 (1989 ).
8.
Noda
I.
, Appl. Spectrosc. 44 , 550 (1990 ).
9.
Noda
I.
, Anal. Chem. 21 , 1065A (1994 ).
10.
Dowrey
A. E.
Hilebrand
G. G.
and
Noda
I
, and C. Marcott, SPIE Proc. 1145 , 156 (1989 ).
11.
Palmer
R. A.
Christopher
C. J.
Manning
J.
Chao
J. L.
Noda
I.
Dowrey
A. E.
and
Marcott
C
, Appl. Spectrosc. 45 , 12 (1991 ).
12.
Gregoriou
V. G.
Noda
I.
Dowrey
A. E.
Marcott
C.
Chao
J. L.
and
Palmer
R. A
, J. Polym. Sci.,Polym. Phys. Ed. 31 , 1769 (1993 ).
13.
Singhal
A.
and
Fina
L. J
, Appl. Spectrosc. 49 , 1073 (1995 ).
14.
Sonoyama
M.
Shoda
K.
Katagiri
G.
and
Ishida
H
, Appl. Spectrosc. 50 , 377 (1996 ).
15.
Budevska
B. O.
Manning
C. J.
and
Griffiths
P. R
, Appl. Spectrosc. 48 , 1556 (1994 ).
16.
Matsuura
H.
Hasegawa
K.
and
Miyazawa
T
, Spectrochim. Acta 42A , 1181 (1986 ).
17.
Tsukada
M.
, J. Polym. Sci., Polym. Phys. Ed. 24 , 1227 (1986 ).
18.
Tsukada
M.
Gotoh
Y.
Nagura
M.
Minoura
N.
Kasai
N.
and
Freddi
G
, J. Polym. Sci., Polym. Phys. Ed. 32 , 961 (1994 ).