Abstract
A simple flow injection chemiluminescence (CL) method for the determination of progesterone and hydrocortisones (HCRs) is described. The method is based on their sensitizing effect on the chemiluminescence reaction of a mercapto-group (-SH) with cerium(IV) in sulfuric acid. This method allows determination in the ranges 0.28–8.0 μg/mL for hydrocortisones (HCRs) and 0.5–8.0 μg/mL for progesterone with a detection limit of 0.10 μg/mL for HCRs and 0.26 μg/mL for progesterone. The experimental conditions for the reaction are optimized. The possible reaction mechanism is discussed.
Get full access to this article
View all access options for this article.
References
1.
Williams
P.A.
Biehl
E. R.
, J. Pharm. Sci. 70 , 530 (1981 ).
2.
Scott
N. R.
Chakraborty
J.
Marks
V.
, Anal. Biochem. 108 , 266 (1980 ).
3.U.S. Pharmacopoeia, United States Pharmacopoeial Convention (Mack , Easton, Pennsylvania , 1985 ), 21st ed.
4.British Pharmacopoeia (HM Stationery Office , London , 1980 ).
5.
Mohamed
A. S.
Mohamed
F. A.
Hassan
H. A.
Selim
N. M.
, Anal. Lett. 20 , 1256 (1987 ).
6.
De Boever
J.
Kohen
F. R.
Serreyn
D
Vandekerckhove
Van Maele
G.
, Anal. Chim. Acta 170 , 125 (1985 ).
7.
Zhang
X. R.
Baeyens
W. R. G.
Van der Weken
G.
Calokerinos
A. C.
Imai
K.
, Anal. Chim. Acta 303 ,137 (1995 ).
8.
Syropoulos
A. B.
Sarantonis
E. G.
Calokerinos
A. C.
, Anal. Chim. Acta 239 , 195 (1990 ).
9.
Koukli
I. I.
Calokerinos
A. C.
, Analyst 115 , 1553 (1990 ).
10.
Koukli
I. I.
Sarantonis
E. G.
Calokerinos
A. C.
, Analyst 113 , 603 (1988 ).
11.
Koukli
I. I.
Sarantonis
E. G.
Calokerinos
A. C.
, Anal. Lett. 23 , 1167 (1990 ).