Sage Journals: Discover world-class research

Abstract

The effects of a heavy-atom salt on the room-temperature solid-matrix luminescence properties of the (±)-trans-7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene-DNA adducts [(±)-anti-BPDE-DNA adducts] and benzo[a]pyrene-r-7,t-8,9,c-10-tetrahydrotetrol (tetrol I-1) on several solid matrices were investigated. TlNO₃ was found to be very effective in enhancing the room-temperature phosphorescence of the (±)-anti-BPDE-DNA adducts and tetrol I-1. However, the room-temperature fluorescence signals were quenched from the (±)-anti-BPDE-DNA adducts and tetrol I-1 on the solid matrices with the heavy-atom salt. It was found that 1% α-cyclodextrin/TlNO₃, 10% trehalose/TlNO₃, and 30% TlNO₃/sodium acetate (NaOAc) yielded very strong RTP signals from the (±)-anti-BPDE-DNA adducts. The greatest enhancement of the RTP intensities from the (±)-anti-BPDE-DNA adducts was achieved with 30% TlNO₃/NaOAc. In addition, a very low limit of detection (0.14 fmole/mg) for tetrol I-1 was acquired with 30% TlNO₃/NaOAc. With its tremendous enhancement of RTP signals from the adducts and tetrol I-1 and the sub-femtomole limit of detection obtained for tetrol I-1, 30% TlNO₃/NaOAc is ideal for the characterization and analysis of DNA adducts and their tetrol products.

Keywords

Cyclodextrin Heavy-atom effect Sodium acetate Solid-matrix luminescence Tetrols Trehalose

Get full access to this article

View all access options for this article.

References

Grimmer

and Pott

, in Environmental Carcinogenesis: Polycyclic Aromatic Hydrocarbons, Grimmer

, Ed. (CRC Press, Boca Raton, Florida, 1983), pp. 84–101.

Grimmer

and Pott

, in Environmental Carcinogenesis: Polycyclic Aromatic Hydrocarbons, Grimmer

, Ed. (CRC Press, Boca Raton, Florida, 1983), pp. 101–113.

De Flora

Zanacchi

Bennicelli

Camoirano

Basso

Bagnasco

Izzotti

and Badolati

G. S

, in Carcinogenic, Mutagenic, and Teratogenic Marine Pollutants: Impact on Human Health and the Environment , Advances in Applied Biotechnology Series Vol. 5, Asvall

J. E.

, Ed. (GPC, Houston, Texas, 1989), pp. 3–31.

Grunberger

and Weinstein

I. B

, in Chemical Carcinogens and DNA, Grover

P. L.

, Ed. (CRC Press, Boca Raton, Florida, 1979), Vol. 2, pp. 59–93.

Estabrook

R. W.

Werringloer

Capdevila

and Prough

R. A

, in Polycyclic Hydrocarbons and Cancer, Gelboin

H. V.

and Ts'o

P. O

, Eds. (Academic Press, New York, 1978), Vol. 1, pp. 285–319.

Ishimura

Iizuka

Morishima

and Hayaishi

, in Polycyclic Hydrocarbons and Cancer, Gelboin

H. V.

and Ts'o

P. O

, Eds. (Academic Press, New York, 1978), Vol. 1, p. 321–333.

Conney

A. H.

, Cancer Res. 42, 4875 (1982).

Geacintov

N. E.

, in Polycyclic Aromatic Hydrocarbon Carcinogenesis: Structure–Activity Relationships, Yang

S. K.

and Silverman

B. D

, Eds. (CRC Press, Boca Raton, Florida, 1988), Vol. 2, pp. 181–206.

Grunberger

and Theall

, in Human Carcinogenesis, Harris

C. C.

and Autrup

H. N

, Eds. (Academic Press, New York, 1983), pp. 195–216.

10.

Autrup

and Harris

C. C

, in Human Carcinogenesis, Harris

C. C.

and Autrup

H. N

, Eds. (Academic Press, New York, 1983), pp. 169–194.

11.

Singer

and Drunberger

, Molecular Biology of Mutagens and Carcinogens (Plenum Press, New York, 1983).

12.

Sanders

M. J.

Cooper

R. S.

Jankowiank

and Small

G. J

, Anal. Chem. 58, 816 (1986).

13.

Kolubayev

Brenner

H. C.

and Geacintov

N. E

, Biochem. 26, 2638 (1987).

14.

Weston

Rowe

M. L.

Manchester

D. K.

Farmer

P. B.

Mann

D. L.

and Harris

C. C

, Carcinogenesis 10, 251 (1989).

15.

Zhao

Liu

T. M.

Kim

S. K.

Macleod

M. C.

and Geacintov

N. E

, Carcinogenesis 13, 1817 (1992).

16.

Wang

and O'Laughlin

J. W

, Environ. Sci. Technol. 26, 2294 (1992).

17.

Vo-Dinh

, Room Temperature Phosphorimetry for Chemical Analysis (Wiley, New York, 1984).

18.

Hurtubise

R. J.

, Phosphorimetry: Theory, Instrumentation, and Applications (VCH, New York, 1990).

19.

Richmond

M. D.

and Hurtubise

R. J

, Anal. Chem. 61, 2643 (1989).

20.

Corley

J. S.

and Hurtubise

R. J

, Anal. Chem. 65, 2601 (1993).

21.

Chu

and Hurtubise

R. J

, Anal. Lett. 26, 1195 (1993).

22.

Chu

and Hurtubise

R. J

, Photochem. and Photobiol. 59, 603 (1994).

23.

Chu

and Hurtubise

R. J

, Photochem. and Photobiol. 62, 251 (1995).

24.

Corley

and Hurtubise

R. J

, Appl. Spectrosc. 48, 747 (1994).

25.

Von Wandruszka

R. M. A.

and Hurtubise

R. J

, Anal. Chem. 49, 2164 (1977).

26.

Maniatis

Fritsch

E. F.

and Sambrook

, Molecular Cloning: A Laboratory Manual (CSH Press, New York, 1982).

27.

Weston

, Mutation Res. 288, 19 (1993).

28.

Ramasamy

S. M.

and Hurtubise

R. J

, Anal. Chem. 59, 2144 (1987).

29.

Barltrop

J. A.

and Coyle

J. D

, Principles of Photochemistry (John Wiley and Sons, New York, 1978), pp. 82–83.

30.

Purdy

B. B.

and Hurtubise

R. J

, Appl. Spectrosc. 46, 988 (1992).

31.

Long

G. L.

and Winefordner

J. D

, Anal. Chem. 55, 712A (1983).

Effects of a Heavy-Atom Salt on the Solid-Matrix Luminescence Properties of the (±)- Trans -7,8-Dihydroxy- Anti -9,10-Epoxy-7,8,9,10-Tetrahydrobenzo[ a ]Pyrene-DNA Adducts and Tetrol I-1 Adsorbed on Several Solid Matrices

Abstract

Keywords

Get full access to this article

References