Abstract
An ultrafast laser apparatus has been developed that allows time-resolved UV-visible and infrared spectroscopy to be performed with a time resolution of 200 fs. The instrument design allows UV-vis and IR data to be collected either as spectra or as kinetic profiles. Solution flow and cell translation sampling systems have been developed that allow the handling of chemical and biochemical systems, including highly air-sensitive materials and volumes as small as 50 μL. The apparatus performance has been evaluated with the use of trans-stilbene in hexane (UV-vis) and silicon (IR) test samples, and the techniques have been applied in combination to study both native carbonmonoxymyoglobin and the novel H64L mutant.
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References
1.Time-resolved Spectroscopy ,
Clark
R. J. H.
and
Hester
R. E.
, Eds. (Wiley , Chichester , 1989 ).
2.
Poliakoff
M.
and
Weitz
E.
, Adv. Organomet. Chem. 25 , 277 (1986 ).
3.
George
M. W.
Poliakoff
M.
, and
Turner
J. J.
, Analyst 119 , 551 (1994 ).
4.
Iwata
K.
and
Hamaguchi
H.
, Appl. Spectrosc. 44 , 1431 (1990 ).
5.
Yuzawa
T.
Kato
C.
George
M. W.
, and
Hamaguchi
H.
, Appl. Spectrosc. 48 , 684 (1994 ).
6.
Uhmann
W.
Becker
A.
Taran
C.
, and
Siebert
F.
, Appl. Spectrosc. 45 , 390 (1991 ).
7.
Manning
C. J.
Palmer
R. A.
, and
Chao
J. L.
, Rev. Sci. Instrum. 62 , 1219 (1991 ).
8.
Masutani
K.
Sugisawa
H.
Yokota
A.
Furukawa
Y.
, and
Tasumi
M.
, Appl. Spectrosc. 46 , 560 (1992 ).
9.
Alawi
S. M.
Krug
T.
, and
Richardson
H. H.
, Appl. Spectrosc. 47 , 1626 (1993 ).
10.Special issue , Appl. Spectrosc. 47 (9 ) (1993 ).
11.
Bensasson
R. V.
Land
E. J.
, and
Truscott
T. G.
, Flash Photolysis and Pulse Radiolysis (Pergamon , Oxford , 1983 ).
12.
Fleming
G. R.
, Chemical Applications of Ultrafast Spectroscopy (Oxford University Press , Oxford , 1986 ).
13.
Anfinrud
P. A.
Johnson
C. K.
Sension
R.
, and
Hochstrasser
R. M.
, in Applied Laser Spectroscopy ,
Andrews
D. L.
, Ed. (VCH , Weinheim , 1992 ), Chap. 10, p. 401 .
14.Ultrafast Dynamics of Chemical System ,
Simon
J. D.
, Ed. (Kluwer Academic , Dordrecht , 1994 ).
15.
Simon
J. D.
, Rev. Sci. Insrum. 60 , 3597 (1989 ).
16.Ultrafast Phenomena VIII ,
Martin
J. L.
Migus
A.
Morurou
G.
, and
Zewail
A. H.
, Eds. (Springer-Verlag , Berlin , 1993 ).
17.
Moore
J. N.
Hansen
P. A.
, and
Hochstrasser
R. M.
, Chem. Phys. Lett. 138 , 110 (1987 ).
18.
Anfinrud
P. A.
Han
C. H.
Lian
T.
, and
Hochstrasser
R. M.
, J. Phys. Chem. 95 , 574 (1991 ).
19.
Locke
B.
Diller
R.
, and
Hochstrasser
R. M.
, in Biomolecular Spectroscopy B ,
Clark
R. J. H.
and
Hester
R. E.
, Eds. (Wiley , Chichester , 1993 ), Chap. 1, p. 1 .
20.
Stoutland
P. O.
Dyer
R. B.
, and
Woodruff
W. H.
, Science 257 , 1913 (1992 ).
21.
Dougherty
T. P.
and
Heilweil
E. J.
, Opt. Lett. 19 , 129 (1994 ).
22.
Jedju
T. M.
Robertson
M. W.
, and
Rothberg
L.
, Appl. Opt. 31 , 2684 (1992 ).
23.
Hamm
P.
Wiemann
S.
Zurek
M.
, and
Zinth
W.
, Opt. Lett. 19 , 1642 (1994 ).
24.
Atkinson
G. H.
Brack
T. L.
Blanchard
D.
, and
Rumbles
G.
, Chem. Phys. 131 , 1 (1989 ).
25.
Weaver
W. L.
Huston
L. A.
Iwata
K.
, and
Gustafson
T. L.
, J. Phys. Chem. 96 , 8956 (1992 ).
26.
Iwata
K.
Yamaguchi
S.
, and
Hamaguchi
H.
, Rev. Sci. Instrum. 64 , 2140 (1993 ).
27.
Matousek
P.
Hester
R. E.
Moore
J. N.
Parker
A. W.
Phillips
D.
Toner
W. T.
Towrie
W.
Turcu
I. C. E.
, and
Umapathy
S.
, Meas. Sci. Technol. 4 , 1090 (1993 ).
28.
Hebert
P.
Marguet
S.
Gustavsson
T.
, and
Mialocq
J. C.
, Optics Comm. 90 , 85 (1992 ).
29.
van Hoek
A.
Bebelaar
D.
, and
Visser
A. J. W. G.
, Appl. Opt. 32 , 3217 (1993 ).
30.
Waldeck
D. H.
, Chem. Rev. 91 , 415 (1991 ).
31.
Greene
B. I.
Hochstrasser
R. M.
, and
Weisman
R. B.
, Chem. Phys. Lett. 62 , 427 (1979 ).
32.
Saltiel
J.
and
Sun
Y. P.
, J. Phys. Chem. 93 , 6246 (1989 ).
33.
Myers
A. B.
Holt
P. L.
Pereira
M. A.
, and
Hochstrasser
R. M.
, Chem. Phys. Lett. 132 , 585 (1986 ).
34.
Courtney
S. H.
Kim
S. K.
Canonica
S.
, and
Fleming
G. R.
, J. Chem. Soc., Faraday Trans. 282 , 2065 (1986 ).
35.
Jedju
T. M.
and
Rothberg
L.
, Appl. Opt. 27 , 615 (1988 ).
36.
Petrich
J. W.
and
Martin
J. L.
, Chem. Phys. 131 , 31 (1989 ).
37.
Springer
B. A.
Sligar
S. G.
Olson
J. S.
, and
Phillips
G. N.
, Chem. Rev. 94 , 699 (1994 ).
38.
Anfinrud
P. A.
Han
C.
, and
Hochstrasser
R. M.
, Proc. Natl. Acad. Sci. USA 86 , 8387 (1989 ).
39.
Anderton
C. L.
Dukic
D.
, and
Moore
J. N.
, unpublished work.