Determination of Chlorophyll a and Pheophytin a Concentrations in Acetone Extracts by Laser Fluorometry

Abstract

We have used the technique of laser fluorometry for chlorophyll a and pheophytin a measurements in 90% acetone/water extracts of sea-water phytoplankton. In our method, the acetone Raman signal is used as an internal standard. We have shown that use of the second harmonic of a pulsed Nd:YAG laser (λ = 532 nm) makes it possible to measure pigment concentrations as low as 10 ng/L. It creates favorable conditions for high-efficiency analysis of the phytoplankton pigments in the oligotrophic areas of the world ocean and for investigations of the feed process of individual zooplankton samples.

Keywords

Chlorophyll Pheophytin Acetone extract Laser fluorometer Phytoplankton Zooplankton

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