The detection and quantitation of the tetrols are important ways to study the extent of chemical damage to DNA. Both room- and low-temperature laser-excited solution fluorescence spectra of the four tetrol isomers were compared for their spectral features. The results showed that the characteristics of the solution spectra obtained at 77 K provided a very reliable means for distinguishing tetrol 1–2 from its isomers. By utilizing both room-temperature solid-matrix (10% α-cyclodextrin/NaCl) fluorescence spectra from earlier work and solution florescence spectra at 77 K obtained in this work, it is possible to identify the four tetrol isomers.
GeacintovN. E., in Polycyclic Aromatic Hydrocarbon Carcinogenesis Structure-Activity Relationships, YangS. K. and SilvermanB. D., Eds. (CRC Press, Boca Raton, Florida, 1988), Vol. II, pp. 181–206.
2.
Polycyclic Hydrocarbons and Carcinogenesis, HarveyR. G., Ed. (American Chemical Society, Washington, D.C., 1985), Series 283.
3.
JankowiakR. and SmallG. J., Chem. Res. Toxicol.4, 256 (1991).
4.
RojasM.CamusA.-M.AlexandrovK.Husgafvel-PursiainenK.AnttilaS. and VainioH, and BartschH., Carcinogenesis13, 929 (1992).
5.
Vo-DinhT. and UzielM., Anal. Chem.59, 1093 (1987).
6.
JohnsonR. W. and Vo-DinhT., Anal. Chem.61, 2766 (1989).
7.
TjioeS. W. and HurtubiseR. J., Anal. Lett.26, 557 (1993).
8.
CorleyJ. S. and HurtubiseR. J., Anal. Chem.65, 2601 (1993).
9.
RichmondM. D. and HurtubiseR. J., Anal. Chim. Acta.255, 335 (1991).
10.
CorleyJ. S. and HurtubiseR. J., Anal. Lett.25, 1559 (1992).
11.
ShuL. and HurtubiseR. J., Appl. Spectrosc.47, 1892 (1993).
12.
ShugartL.McCarthyJ. and JimenezB, and DanielsJ., Aquatic Toxicol.9, 319 (1987).
