Abstract
Surface-enhanced resonance Raman spectroscopy (SERRS) of riboflavin, flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) on Cu, Cu-Zn-Pb, and Fe as well as Fe-C-Cr-Ni has been performed by means of the modification of these substrates with silver nitrate. It is found that the SERR spectrum of a flavin molecule on the alloy Cu-Zn-Pb is different from that on the pure Cu, and this is also the case for a flavin molecule on the alloy Fe-C-Cr-Ni and on pure Fe. This result indicates possibly that Zn, rather than Cu, and Ni or Cr, rather than Fe, in the alloys also interact with the flavin molecules. In addition, the three flavins on the same supporting metal surfaces also exhibit some differences in the SERR spectra. This result implies an involvement of the side chains of the flavin molecules in the enhancement. The SERR spectra of the three flavins are compared with their resonance Raman (RR) spectra. On the basis of our experimental results, a direct interaction of N–5 and possibly also O–4 in the flavins with silver is suggested. The general effectiveness of this method for obtaining SERS is demonstrated, and the enhancement mechanism of these substrates is also briefly discussed.
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References
1.
Moskovits
M.
, Rev. Mod. Phys. 57 , 783 (1985 ).
2.Surface Enhanced Raman Scattering ,
Furtak
T. E.
and
Chang
R. K
, Eds. (Plenum Press , New York , 1982 ).
3.
Otto
A.
, in Light Scattering in Solids ,
Cardona
M.
and
Güntherodt
G
, Eds. (Springer-Verlag , Berlin , 1984 ) Vol. 4 , p. 259 .
4.
Crookel
A.
Fleischmann
M.
Hanniet
M.
and
Hendra
P. J
, Chem. Phys. Lett. 149 , 123 (1988 ).
5.
Chase
D. B.
and
Parkinson
B. A
, J. Phys. Chem. 95 , 7810 (1991 ).
6.
Angel
S. M.
and
Archibald
D. D
, Appl. Spectrosc. 43 , 1097 (1989 ).
7.
Zhang
P. X.
Wang
X.
and
Guo
Z. G
, Chinese Phys. Lett. 7 , 465 (1990 ).
8.
Liang
E. J.
Engert
C.
and
Kiefer
W
, J. Raman Spectrosc. 24 , 775 (1993 ).
9.
Moskovits
M.
and
DiLella
D. P
, in Surface Enhanced Raman Scattering ,
Furtak
T. E.
and
Chang
R. K
, Eds. (Plenum Press , New York , 1982 ), p. 243 .
10.
Lund
P. A.
Smardzewski
R. R.
and
Tevault
D. E
, Chem. Phys. Lett. 89 , 508 (1982 ).
11.
Schulte
W.
Breithaupt
B.
Charle
K. P.
and
Kloss
U
, Ber. Bunsenges. Physik. Chem. 88 , 308 (1984 ).
12.
DiLella
D. P.
and
Zhou
P
, Chem. Phys. Lett. 166 , 240 (1990 ).
13.
Loufty
R. O.
and
Aroca
R
, J. Luminescence 26 , 359 (1982 ).
14.
Feilchenfeld
H.
Gao
X.
and
Weaver
M. J
, Chem. Phys. Lett. 161 , 321 (1989 ).
15.
Gao
Y.
and
Lopez-Rios
T
, Phys. Rev. Lett. 53 , 2853 (1984 ).
16.
Pemberton
J. E.
Coria-Garcia
J. C.
and
Hoff
R. L
, Langmuir. 3 , 150 (1987 ).
17.
Stacy
A. M.
and
VanDuyne
R. P
, Chem. Phys. Lett. 102 , 365 (1983 ).
18.
Guzonas
D. A.
Atkinson
G. F.
and
Irish
D. E
, Chem. Phys. Lett. 107 , 193 (1984 ).
19.
Hutchinson
K.
McQuillan
A. J.
and
Hester
R. E
, Chem. Phys. Lett. 98 , 27 (1983 ).
20.
Siiman
O.
Bumm
L. A.
Callaghan
R.
Blatchford
C. G.
and
Kerker
M
, J. Phys. Chem. 87 , 1014 (1983 ).
21.
Lee
P. C.
and
Meisel
D
, Chem. Phys. Lett. 99 , 262 (1983 ).
22.
Wetzel
H.
and
Gerischer
H
, Chem. Phys. Lett. 76 , 4640 (1980 ).
23.
Moskovits
M.
and
DiLella
D. P
, J. Chem. Phys. 73 , 6068 (1980 ).
24.
Goudonnet
J. P.
Begun
G. M.
and
Arakawa
E. T
, Chem. Phys. Lett. 92 , 197 (1982 ).
25.
Vo-Dinh
T.
Meier
M.
and
Wokaun
A
, Anal. Chim. Acta 181 , 139 (1986 ).
26.
Vo-Dinh
T.
Hiromoto
M. Y. K.
Begun
G. M.
and
Moody
R. L
, Anal. Chem. 56 , 1667 (1984 ).
27.
Alak
A. M.
and
Vo-Dinh
T
, Anal. Chem. 61 , 656 (1989 ).
28.
Bello
J. M.
Stokes
D. L.
and
Vo-Dinh
T
, Anal. Chem. 61 , 1723 (1989 ).
29.
Bello
J. M.
Stokes
D. L.
and
Vo-Dinh
T
, Appl. Spectrosc. 43 , 1325 (1989 ).
30.
Liang
E. J.
and
Zhang
P. X
, J. Light Scattering 1 , 35 (1989 ).
31.
Li
Y.-S.
and
Wang
Y
, Appl. Spectrosc. 46 , 142 (1992 ).
32.
Pagannone
M.
Quagliano
L. G.
Mattioli
L.
and
Mattei
G
, J. Raman Spectrosc. 22 , 825 (1991 ).
33.
Xue
Q.
Dong
J.
and
Zhang
M
, Appl. Spectrosc. 45 , 756 (1991 ).
34.
Liu
S. W.
and
Zhang
P. X
, unpublished research.
35.
Liao
P. F.
Bergman
J. G.
Chemla
D. S.
Wokaun
A.
Melngailis
J.
Hawryluk
A. M.
and
Economou
N. P
, Chem. Phys. Lett. 82 , 355 (1981 ).
36.
Roth
E.
Hope
G. A.
Schweinsberg
D. P.
Kiefer
W.
and
Fredericks
P. M
, Appl. Spectrosc. 47 , 1794 (1993 ).
37.
Nishimura
Y.
and
Tsuboi
M
, Chem. Phys. Lett. 59 , 210 (1978 ).
38.
Dutta
P. K.
Nestor
J.
and
Spiro
T. G
, Biochem. Biophys. Res. Commun. 83 , 209 (1978 ).
39.
Brakel
R.
and
Schneider
F. W
, in Advances in Nonlinear Spectroscopy ,
Clark
R. J. H.
and
Hester
R. E
, Eds. (John Wiley , Chichester , 1988 ), Vol. 15 , p. 149 .
40.
Benecky
M.
Li
T. Y.
Schmidt
J.
Freiman
F.
Watters
K. L.
and
McFarland
J. T
, Biochemistry 18 , 3471 (1979 ).
41.
Cotton
T. M.
and
Holt
R
, in Spectroscopy of Biological Molecules ,
Alix
A. J. P.
Bernard
L.
and
Manfait
M
, Eds. (John Wiley , New York , 1985 ) p. 38 .
42.
Xu
J.
Birke
R. L.
and
Lombardi
J. R
, J. Am. Chem. Soc. 109 , 5645 (1987 ).
43.
Lee
N.-S.
Sheng
R.-S.
Morris
M. D.
and
Schopfer
L. M
, J. Am. Chem. Soc. 108 , 6179 (1986 ).
44.
Liang
E. J.
and
Zhang
P. X
, Acta Phys. Sinica 40 , 198 (1991 ).
45.
Hansma
P. K.
, Phys. Report 30c , 145 (1977 ).
46.
Rubim
J. C.
, Chem. Phys. Lett. 167 , 209 (1990 ).
47.
Ahern
A. M.
and
Garrell
R. L
, Anal. Chem. 59 , 2816 (1987 ).
48.
Liang
E. J.
Wang
Y. T.
Wang
Y. J.
Hao
Q. X.
and
Zhang
P. X
, Chinese Phys. Lett. 9 , 73 (1992 ).
49.
Bowman
W. D.
and
Spiro
T. G
, Biochemistry 20 , 3313 (1981 ).
50.
Weitz
D. A.
Garoff
S.
Gersten
J. I.
and
Nitzan
A
, J. Chem. Phys. 78 , 5324 (1983 ).
51.
Schmidt
J.
Coudron
Ph.
Thompson
A. W.
Watters
K. L.
and
McFarland
T
, Biochemistry 22 , 76 (1983 ).
52.
Abe
M.
and
Kyogoku
Y
, J. Phys. Chem. 43 , 1027 (1987 ).
53.
Nishina
Y.
Kitagawa
T.
Shiga
K.
Horiike
K.
Matsumura
Y.
Watari
H.
and
Yamano
T
, J. Biochem. (Tokyo) 84 , 925 (1978 ).
54.
Kitagawa
T.
Nishina
Y.
Kyogoku
Y.
Yamano
T.
Ohishi
N.
Takai-Suzuki
A.
and
Yagi
K
, Biochemistry 18 , 1804 (1979 ).
55.
Dutta
P. K.
and
Spiro
T. G
, Biochemistry 19 , 1590 (1980 ).
56.
Dutta
P. K.
Spencer
R.
Walsh
C.
and
Spiro
T. G
, Biochim. Biophys. Acta 623 , 77 (1980 ).
57.
Benecky
M.
Yu
T.-J.
Watters
K. L.
and
McFarland
J. T
, Biochim. Biophys. Acta 626 , 197 (1980 ).
58.
Benecky
M. J.
Dowling
M. G.
Clark
M. J.
and
Spiro
T. G
, Inorg. Chem. 23 , 865 (1984 ).
59.
Morris
M. D.
and
Bienstock
R. J
, in Spectroscopy of Biological Systems ,
Clark
R. J. H.
and
Hester
R. E
, Eds. (John Wiley , New York , 1986 ), pp. 395 –442 .