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Abstract

The interaction between hematoporphyrin and guanine has been investigated by the Raman spectroscopic method. Irradiation of guanine solution mixed with hematoporphyrin was observed through the change in the Raman spectra. It was observed that the band at 1534 cm⁻¹ shifted and the bands at 1274 cm⁻¹ and 1190 cm⁻¹ disappeared in the presence of hematoporphyrin. A prolonged irradiation time of five hours caused the SERS spectra of guanine to disappear. These results indicate that the guanine was modified in the photosensitized reactions in the presence of hematoporphyrin. We used SERS to study the photodynamic modification of nucleic acid base by hematoporphyrin. We found that illumination of guanine and adenine with the 514.5-nm argon-ion laser line in the presence of hematoporphyrin resulted in the selected degradation of guanine.

Keywords

Raman spectroscopy Guanine-hematoporphyrin interaction

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References

Pasternack

R. F.

Gibbs

E. J.

and Villafranca

J. J

, Biochemistry 22, (1983).

Kelly

J. M.

Murphy

M. J.

McConnell

D. J.

and OhUigin

, Nucleic Acids Res. 13, 167 (1985).

Marzilli

L. G.

Banville

D. L.

Zon

and Wilson

W. D

, J. Am. Chem. Soc. 108, 4188 (1986).

Strickland

J. A.

Banville

D. L.

Wilson

W. D.

and Marzilli

L. G

, Inorg. Chem. 36, 3398 (1987).

Dougherty

Pilbrow

J. R.

Skorobogaty

and Smith

T. D

, J. Chem. Soc. Faraday Trans. 2, 81, 1739 (1985).

Geacintov

N. E.

Ibanez

Rougee

and Beansasson

R. V

, Biochemistry 26, 3087 (1987).

Bromley

S. D.

Ward

B. W.

and Dabrowiak

J. C

, Nucleic Acids Res. 14, 9133 (1986).

A. T.

, Raman Spectroscopy in Biology: Principles and Applications (John Wiley, New York, 1982).

Nishimura

Tsuboi

Kato

and Morokuma

, Chem. Soc. Japan 58, 638 (1985).

10.

Nishimura

Tsuboi

Kato

and Morokuma

, Amer. Chem. Soc. 103, 1354 (1981).

11.

Delabar

J. M.

and Majoube

, Spectrochim. Acta 34A, 129 (1978).

12.

Mail

R. A.

, Biochim. Biophys. Acta 120, 461 (1966).

13.

Koglin

Sequaris

J. M.

and Valenta

, J. Mol. Struct. 60, 421 (1980).

14.

Otto

DeMul

F. F. M.

Huizinga

and Greve

, J. Phys. Chem. 92, 1239 (1988).

15.

Suh

J. S.

and Moskovits

, J. Am. Chem. Soc. 108, 4711 (1986).

16.

Dubbelman

T. M. A. R.

Van Steveninck

A. L.

and Van Steveninck

, Biochim. Biophys. Acta 719, 47 (1982).

17.

Simon

M. I.

and Van Vunakis

, Arch. Biochem. Biophys. 105, 197 (1964).

18.

Sussenbach

J. S.

and Berends

, Biochim. Biophys. Acta 76, 154 (1963).

19.

Nouen

D. L.

Marko

Vermeersch

Febvay-Garot

Lablache-Combier

Perree-Fauvet

and Gaudemer

, Photochem. Photobiol. 49, 7 (1989).

20.

Gutter

Speck

W. T.

and Rosenkranz

H. S

, Biochim. Biophys. Acta 475, 307 (1977).

21.

Houba-Herin

Calberg-Baco

C. M.

Piette

and Vorst

A. V

, Photochem. Photobio. 36, 297 (1982).

Surface-Enhanced Raman Scattering (SERS) Study of the Interaction between Hematoporphyrin and Guanine

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