Abstract
Troponin C (TnC) is covalently labeled with a fluorescent probe molecule dansylaziridine and immobilized on polyethyleneimine-impregnated cellulose. An optrode is constructed by attaching the treated cellulose membrane to the distal end of a bifurcated fiber-optic cable. Because fluorescence is enhanced as Ca+2 binds with the immobilized dansylated troponin (TnCDANZ), this system can be used as a calcium sensor for monitoring plasma Ca+2 levels.
Keywords
Get full access to this article
View all access options for this article.
References
1.
Borman
S. A.
, Anal. Chem. 53 , 1616A (1981 ).
2.
Saari
L. A.
and
Seitz
W. R
, Anal. Chem. 54 , 821 (1982 ).
3.
Saari
L. A.
and
Seitz
W. R
, Anal. Chem. 55 , 667 (1983 ).
4.
Vo-Dinh
T.
Tromberg
B. J.
Griffin
G. D.
Ambrose
K. R.
Sepaniak
M. J.
and
Gardenhire
E. M
, Appl. Spectrosc. 41 , 735 (1987 ).
5.
Tromberg
B. J.
Eastham
J. F.
and
Sepaniak
M. J
, Appl. Spectrosc. 38 , 38 (1984 ).
6.
Scheper
T.
and
Buckmann
A. F
, Biosensors and Bioelectronics 5 , 125 (1990 ).
7.
Tromberg
B. J.
Sepaniak
M. J.
Vo-Dinh
T.
and
Griffin
G. D
, Anal. Chem. 59 , 1226 (1987 ).
8.
Ebashi
S.
and
Endo
M
, Prog. Biophys. Mol. Biol. 18 , 123 (1968 ).
9.
Inesi
G.
, Annu. Rev. Biophys. Bioeng. 1 , 191 (1972 ).
10.
Tada
M.
Yamamoto
T.
and
Tonomura
Y
, Physiol. Rev. 58 , 1 (1978 ).
11.
Johnson
J. D.
Collins
J. H.
and
Potter
J. D
, J. Biol. Chem. 253 , 6451 (1978 ).
12.
Iio
T.
and
Kondo
H
, J. Biochem. 88 , 547 (1980 ).
13.
Iio
T.
and
Kondo
H
, J. Biochem. 90 , 163 (1981 ).
14.
Ingraham
R. H.
and
Swenson
C. A
, Biochemistry 24 , 5221 (1985 ).
15.
Johnson
J. D.
and
Schwartz
A
, J. Biol. Chem. 253 , 5243 (1978 ).
16.
Lane
Helen
, in a paper presented at the American Association for Clinical Chemist Conference on Clinical Chemistry in Space, Houston, Texas (1990 ).
17.
Suszu
A.
, Chem. Abst. 78 , 137416v (1973 ).
18.
Udenfriend
Sidney
, Fluorescence Assay in Biology and Medicine (Academic Press , New York , 1962 ), Vol. I , pp. 106 –108 .