Abstract
Acquisition times as short as 10 ms are achieved for the collection of fluorescent decays with a time-correlated single-photon counting detection scheme, arranged in the reverse-interactive configuration, and high-repetition-rate laser excitation. Faster acquisition times for the measurement of fluorescent decays are accomplished by employing the same laser excitation system and photomultiplier anode-current sampling with a digital oscilloscope. With this variant, an 8.9 ns exponential decay is collected in 8.058
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References
1.
Simpson
R. C.
, in High Performance Liquid Chromatography ,
Brown
P. R.
and
Hartwick
R. A
, Eds. (John Wiley and Sons , New York , 1989 ), Chap. 9.
2.
Wu
S.
and
Dovichi
N. J
, J. Chromatogr. 480 , 141 (1989 ).
3.
Dickson
P. N.
and
Margerum
D. W
, Anal. Chem. 58 , 3153 (1986 ).
4.
Elzinga
P. A.
Kneisler
R. J.
Lytle
F. E.
Jiang
Y.
King
G. B.
and
Laurendeau
N. M
, Appl. Opt. 26 , 4303 (1987 ).
5.
Dovichi
N. J.
Martin
J. C.
Jett
J. H.
Trkula
M.
and
Keller
R. A
, Anal. Chem. 56 , 348 (1984 ).
6.
Lytle
F. E.
, J. Chem. Ed. 59 , 915 (1982 ).
7.
Gustafson
T. L.
and
Lytle
F. E
, Appl. Spectrosc. 34 , 185 (1980 ).
8.
Ramsey
J. M.
Heiftje
G. M.
and
Haugen
G. R
, Appl. Opt. 18 , 1913 (1979 ).
9.
Harris
T. D.
and
Lytle
F. E
, in Ultrasensitive Laser Spectroscopy ,
Kliger
D. S.
Ed. (Academic Press , New York , 1983 ), Chap. 7.
10.
Coburn
J. T.
Lytle
F. E.
and
Huber
D. M
, Anal. Chem. 58 , 1123 (1986 ).
11.
Desilets
D. J.
Coburn
J. T.
Lantrip
D. A.
Kissinger
P. T.
and
Lytle
F. E
, Anal. Chem. 58 , 1123 (1986 ).
12.
Alfano
A. J.
, Appl. Opt. 23 , 5010 (1989 ).
13.
Cobb
W. T.
and
McGown
L. B
, Appl. Spectrosc. 43 , 1363 (1989 ).
14.
Haugen
G. R.
and
Lytle
F. E
, Anal. Chem. 53 , 1554 (1981 ).
15.
Nowak
S. A.
and
Lytle
F. E
, Appl. Spectrosc. 45 , 728 (1991 ).
16.
Harris
J. M.
Lytle
F. E.
and
McCain
T. C
, Anal. Chem. 48 , 2095 (1976 ).
17.
Harris
J. M.
Barnes
W. T.
Gustafson
T. H.
Bushaw
T. H.
and
Lytle
F. E
, Rev. Sci. Instrum. 51 , 988 (1980 ).
18.
Ghiggino
K. P.
Skilton
P. F.
and
Thistlethwaite
P. J
, J. Photochem. 31 , 113 (1985 ).
19.
Sakurovs
R.
and
Ghiggino
K. P
, J. Photochem. 18 , 1 (1982 ).
20.
Drahos
D. J.
Hemming
B. C.
and
McPherson
S
, Biotechnology 4 , 439 (1986 ).
21.
Press
W. H.
Flannery
B. P.
Teukolsky
S. A.
and
Vetterling
W. T
, Numerical Recipes , Cambridge University Press , New York , 1986 ), Chap. 7.
22.
Seitzinger
N. K.
Hughes
K. D.
and
Lytle
F. E
, Anal. Chem. 61 , 2611 (1989 ).
23.
James
D. R.
and
Ware
W. R
, Chem. Phys. Lett. 120 , 455 (1985 ).
24.
Kneisler
R. J.
Lytle
F. E.
Fiechtner
G. J.
Jiang
Y.
King
G. B.
and
Laurendeau
N. M
, Opt. Lett. 14 , 260 (1989 ).