Abstract
A fluorescence spectroscopic system with both picosecond time resolution and submicrometer three-dimensional space resolution has been developed for elucidating the dynamics of inhomogeneous photophysical/photochemical phenomena. This system is based on a confocal laser scanning fluorescence microscope and a time-correlated single photon counting method. Results on demonstration experiments are shown to specify the performance of the system. Characteristics and limitations of the system are discussed.
Keywords
Get full access to this article
View all access options for this article.
References
1.
Kahlow
M. A.
Jarzeba
W.
DuBruil
T. P.
and
Barbara
P. F
, Rev. Sci. Instrum. 59 , 1098 (1988 ).
2.
O'Connor
D. V.
and
Phillips
D
, Time-Correlated Single-Photon Counting (Academic Press , London , 1985 ).
3.
Masuhara
H.
Eura
S.
Fukumura
H.
and
Itaya
A
, Chem. Phys. Lett. 156 , 446 (1989 ).
Masuhara
H.
Ikeda
N.
Miyasaka
H.
and
Mataga
N
, J. Spectrosc. Soc. Japan 31 , 19 (1982 ).
4.
Kinoshita
S.
and
Kushida
T
, Anal. Instrum. 14 , 503 (1985 ).
5.
Masuhara
H.
Mataga
U.
Tazuke
S.
Murao
T.
and
Yamazaki
I
, Chem. Phys. Lett. 100 , 415 (1983 ).
6.
Chang
R. K.
, Ber. Bunsenges. Phys. Chem. 91 , 296 (1987 ).
7.
Shank
C. V.
Yen
R.
and
Hirlimann
C
, Phys. Rev. Lett. 51 , 900 (1983 ).
8.Confocal Microscopy ,
Wilson
T.
Ed. (Academic Press , London , 1990 ).
9.
Puppels
G. J.
De Mul
F. F. M.
Otto
C.
Greve
J.
Robert-Nlcoud
M.
Arndt-Jovin
D. J.
and
Jovin
T. M
, Nature 347 , 301 (1990 ).
10.
Van Den Zegel
M.
Boens
N.
Daems
D.
and
De Schryver
F. C
, Chem. Phys. 101 , 311 (1986 ).
11.
Sasaki
K.
and
Masuhara
H
, Appl. Opt. 30 , 977 (1991 ).
12.
Kawata
S.
Arimoto
A.
and
Nakamura
O
, J. Opt. Soc. Am. A 8 , 171 (1991 ).
13.
Misawa
H.
Koshioka
M.
Sasaki
K.
Kitamura
N.
and
Masuhara
H
, Chem. Lett. 1990 , 1479 (1990 ).
14.
Sasaki
K.
Kawata
S.
and
Minami
S
, Appl. Spectrosc. 40 , 185 (1986 ).
15.
Sasaki
K.
and
Kawata
S
, J. Opt. Soc. Am. A 7 , 513 (1990 ).
