Abstract
Near-infrared laser dyes were studied with the use of conventional fluorescence quenching. A systematic study of the effects of different quenchers and temperature on the fluorescence of near-infrared fluorophores is presented. Differences in the quenching of near-infrared fluorophores were observed for quenching by ionic quenchers. Quenching with ionic and nonionic quenchers led to nonlinear quenching results.
Keywords
Get full access to this article
View all access options for this article.
References
1.
Imasaka
T.
Yoshitake
A.
and
Ishibashi
N
, Anal. Chem. 56 , 1077 (1984 ).
2.
Kawabata
Y.
Imasaka
T.
and
Ishibashi
N
, Talanta 33 , 281 (1986 ).
3.
Sauda
K.
Imasaka
T.
and
Ishibashi
N
, Anal. Chem. 58 , 2649 (1986 ).
4.
Arthurs
E. G.
Bradley
D. J.
and
Roddie
A. G
, Appl. Phys. Lett. 20 , 125 (1972 ).
5.
Bradley
D. J.
, Optoelectronics 6 , 25 (1974 ).
6.
Dempster
D. N.
Morrow
T.
Rankin
R.
and
Thompson
G. F
, Chem. Phys. Lett. 18 , 488 (1973 ).
7.
Kuzmin
V. A.
and
Darmanyan
A. P
, Chem. Phys. Lett. 54 , 159 (1978 ).
8.
Cooper
W.
and
Rome
K. A
, J. Phys. Chem. 78 , 16 (1974 ).
9.
Muenter
A. A.
and
Cooper
W
, Chem. Phys. Lett. 22 , 212 (1973 ).
10.
Fouassier
J. P.
Lougnot
D. J.
and
Faure
J
, Opt. Comm. 18 , 263 (1976 ).
11.
Fletcher
N.
, Appl. Phys. 22 , 227 (1980 ).
12.
Feller
K. H.
Faessler
D.
Hampe
P.
Berndt
K.
Klose
E.
and
Schwarz
P
, Z. Phy. Chem. 266 , 2 (1985 ).
13.
Wolf
D. E.
, Biochemistry 24 , 582 (1985 ).
14.
Demchuk
M. I.
Ishchenko
A. A.
Mikhailov
V. P.
and
Avdeeva
V. I
, Chem. Phys. Lett. 144 , 99 (1988 ).
15.
Lakowitz
J. R.
, Principles of Fluorescence Spectroscopy (Plenum Press , New York , 1983 ).
16.
Eftink
M. R.
and
Ghiron
C. A
, Biochemistry 15 , 672 (1976 ).
17.
West
W.
and
Pearce
S
, J. Phys. Chem. 69 , 1894 (1965 ).