Abstract
Protein adsorption (fluorescein labeled gamma globulin, FITC-IgG) on the surface of poly(styrene) thin-film optical waveguides was fluorescently detected. The time course of adsorption, the FITC-IgG fluorescence spectra, and the surface concentration estimates are presented. The results demonstrate the potential use of polymer optical waveguides as integrated optic evanescent sensors.
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References
1.
Newby
K.
Reichert
W. M.
Andrade
J. D.
, and
Benner
R. E.
, Appl. Opt. 23 , 1812 (1984 ).
2.
Reichert
W. M.
Newby
K.
, and
Andrade
J. D.
, Trans. Ann. Meeting Soc. for Biomat. , San Diego, California (1985 ).
3.
Reichert
W. M.
Ives
J. T.
Suci
P. A.
, and
Hlady
V.
, Appl. Spectrosc. 41 , 636 (1987 ).
4.
Marcuse
D.
, Theory of Dielectric Optical Waveguides (Academic Press , New York , 1974 ).
5.
Rabolt
J. F.
Schlotter
N. E.
Swalen
J. D.
, and
Santo
R.
, J. Polymer Sci. 12 , 1 (1983 ).
6.
Miller
D. R.
Han
O. H.
, and
Bohn
P. W.
, Appl. Spectrosc. 41 , 249 (1987 ).
7.
Rabolt
J. F.
Santo
R.
Schlotter
N. E.
, and
Swalen
J. D.
, IBM J. Res. Develop. 26 , 209 (1982 ).
8.
Swalen
J. D.
Tacke
M.
Santo
R.
Rieckhoff
K. E.
, and
Fischer
J.
, Helv. Chimica Acta 61 , 960 (1978 ).
9.
Lakowicz
J. R.
, Principles of Fluorescence Spectroscopy (Plenum Press , New York , 1983 ).
10.
Hlady
V.
Reinecke
D. R.
, and
Andrade
J. D.
, J. Coll. Interf. Sci. 111 , 555 (1986 ).
11.
Rockhold
S. A.
Quinn
R. D.
Van Wagenen
R. A.
Andrade
J. D.
, and
Reichert
W. M.
, J. Electroanal. Chem. 150 , 261 (1983 ).
12.
Cantarero
L. A.
Butler
J. E.
, and
Osborne
J. W.
, Anal. Biochem. 105 , 375 (1980 ).