Abstract
Benzo[a]pyrene-7,8-diol-9,10 epoxide (BPDE)-DNA adducts have been analyzed by surface-enhanced Raman scattering (SERS) spectrometry. BPDE is the ultimate carcinogenic metabolite of benzo[a]pyrene. The SERS measurements were performed with the use of a silver-coated cellulose material as the SERS-active substrate. The acid hydrolyzed products of BPDE-DNA adducts, DNA, and the BP-tetrols were also analyzed by SERS. The results indicate that SERS has great promise as a tool for detecting BPDE-DNA adducts in biological samples.
Keywords
Get full access to this article
View all access options for this article.
References
1.
Bowman
M. C.
, Handbook of Carcinogens and Hazardous Substances (Marcel Dekker , New York , 1982 ).
2.
Gelboin
H. V.
and
Tso
O. P.
, Polycyclic Hydrocarbons and Cancer (Academic Press , New York , 1978 ).
3.Chemical Carcinogens and DNA ,
Grover
P. L.
, Ed. (CRC Press , Boca Raton, Florida , 1979 ), Vols. 1 and 2 .
4.
Gelboin
H. V.
, Physical Rev. 60 , 1107 (1980 ).
5.
Vahakangas
K.
Haugen
A.
, and
Harris
C.
, Carcinogenesis 6 , 1109 (1985 ).
6.
Rahn
R. O.
Chang
S. S.
Holland
J. M.
, and
Shugart
L. R.
, Biochem. Biophys. Res. Comm. 109 , 262 (1982 ).
7.
Santella
R. M.
Lin
C. D.
Cleveland
W. L.
, and
Weinstein
J. B.
, Carcinogenesis 5 , 373 (1982 ).
8.
Sanders
M. J.
Cooper
R. S.
Jankowialk
R.
Small
G. J.
Heisig
V.
, and
Jeffrey
A. M.
, Anal. Chem. 577 , 1148 (1985 ).
9.
Gupta
R. C.
Reddy
M. V.
, and
Randerath
K.
, Carcinogenesis 3 , 1081 (1982 ).
10.
Vo-Dinh
T.
and
Uziel
M.
, paper to appear in Anal. Chem. (April, 1987 ).
11.
Fleischmann
M.
Hendra
P. J.
, and
McQuillan
A. J.
, Chem. Phys. Lett. 26 , 123 (1974 ).
12.
Jeanmaire
D. L.
and
Van Duyne
R. P.
, J. Electr. Chem. 84 , 1 (1977 ).
13.
Albrecht
M. G.
and
Creighton
J. A.
, J. Am. Chem. Soc. 99 , 5215 .14 (1977 ).
14.Surfaced-Enhanced Raman Scattering ,
Chang
R. K.
and
Furtak
T. E.
, Eds. (Plenum Press , New York , 1982 ).
15.
Moskowitz
M.
, Rev. of Modern Phys. 57 , 783 (1980 ).
16.
Vo-Dinh
T.
Hiromoto
M. Y. K.
Begun
G. M.
, and
Moody
R. L.
, Anal. Chem. 56 , 1667 (1984 ).
17.
Goudonnet
J. P.
Begun
G. M.
, and
Arakawa
E. T.
, Chem. Phys. Lett. 92 , 197 (1982 ).
18.
Meier
M.
Wokaun
A.
, and
Vo-Dinh
T.
, J. Phys. Chem. 89 , 1843 (1985 ).
19.
Buncick
M. C.
Warmack
R. J.
Little
J. W.
, and
Ferrell
T. L.
, Bull. Am. Phys. Soc. 29 , 129 (1984 ).
20.
Enlow
P.
Buncick
M.
Warmack
R. J.
, and
Vo-Dinh
T.
, Anal. Chem. 58 , 119 (1986 ).
21.
Tran
C. D.
, Anal. Chem. 56 , 824 (1984 ).
22.
Jennings
C.
Avoca
R.
Hor
A.
, and
Loufyt
R. V.
, Anal. Chem. 56 , 2033 (1984 ).
23.
Lord
R. C.
and
Thomas
G.
, Spectrochim. Acta 23A , 2551 (1967 ).
24.
Ervin
K. M.
Koglin
E.
Sequaris
J. M.
Valenta
P.
, and
Nurnberg
H. V.
, J. Electroanal. Chem. 114 , 179 (1980 ).
25.
Tobin
M. C.
, Spectrochim. Acta 25A , 1855 (1969 ).
26.
Tsuboi
M.
and
Niahimura
Y.
, Raman Spectrosc. Proc. Int. Conf: 8th , 638 (1982 ).
27.
Sanford
A. A.
, Anal. Chem. 56 , 720 (1984 ).