A dual illuminator suitable for Raman difference spectroscopy is described. The system, which can be readily adapted for use with computer-controlled Raman spectrometers, provides a differential frequency accuracy (ca. ±0.03 cm−1) which is comparable to that of other existing devices. The individual control of the temperature of each sample makes it possible to obtain thermally induced Raman difference spectra, where-by very small frequency shifts associated with changes in temperature can be accurately measured. An example of application to the study of the gel-to-liquid crystal transition of a phospholipid (DPPG) is given.
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