Abstract
The measurement and interpretation of relaxation data on macromolecules present special problems. The required instrumental conditions and extensions of relaxation theory are outlined and illustrated by the analysis of 13C and 1H relaxation data on a small protein (bovine pancreatic trypsin inhibitor).
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References
1.
Ernst
R. R.
and
Anderson
W. A.
, Rev. Sci. Instrum. 37 , 93 (1966 ).
2.
Bloembergen
N.
Purcell
E. M.
, and
Pound
R. V.
, Phys. Rev. 73 , 679 (1948 ).
3.
Redfield
A. G.
, IBM J. Res. 1 , 19 (1957 ).
4.
Woessner
D. E.
, J. Chem. Phys. 36 , 1 (1962 ).
5.
Levine
Y. K.
Partington
P.
, and
Roberts
G. C. K.
, Mol. Phys. 25 , 497 (1973 ).
6.
Noack
F.
, in NMR: Basic Principles and Progress ,
Diehl
P.
, Ed. (Springer-Verlag , Berlin , 1971 ), Vol. 3 , pp. 83 –144 .
7.
Jardetzky
O.
, Life Sci. 22 , 1245 (1978 ).
8.
Cozzone
P. J.
Opella
S. J.
Jardetzky
O.
Berthou
J.
, and
Jolles
P.
, Proc. Natl. Acad. Sci. U.S.A. 72 , 2095 (1975 ).
9.
Ribeiro
A. A.
King
R.
Restivo
C.
, and
Jardetzky
O.
, J. Am. Chem. Soc. 102 , in press (1980 ).
10.
Jardetzky
O.
Ribeiro
A. A.
, and
King
R.
, Biochem. Biophys. Res. Commun. 92 , 883 (1980 ).
11.
Farrar
T. C.
and
Becker
E. D.
, Pulse and Fourier Transform NMR (Academic Press , New York , 1971 ), Ch. 5, pp. 66 –85 .
12.Bruker WP 60/WP 80 Operations Manual , Bruker Instruments, Inc. , Billerica, MA , p. 3 .
13.
Abragam
A.
, The Principles of Nuclear Magnetism (Clarendon Press , Oxford , 1961 )
14.
Gutowsky
H. S.
McCall
D. W.
, and
Slichter
C. P.
, J. Chem. Phys. 21 , 279 (1953 ).
15.
McConnell
H. M.
, J. Chem. Phys. 28 , 430 (1958 ).
16.
Solomon
I.
, Phys. Rev. 99 , 559 (1955 ).
17.
Wangsness
R. K.
and
Bloch
F.
, Phys. Rev. 89 , 728 (1953 ).
18.
Redfield
A. G.
, Adv. Mag. Res. 1 , 1 (1965 ).
19.
Kubo
R.
and
Tomita
K.
, J. Phys. Soc. Japan 9 , 888 (1954 ).
20.
Woessner
D. E.
, J. Chem. Phys. 37 , 647 (1962 ).
21.
Doddrell
D.
Glushko
V.
, and
Allerhand
A.
, J. Chem. Phys. 56 , 3683 (1972 ).
22.
Levine
Y. K.
Birdsall
N. J. M.
Lee
A. G.
Metcalfe
J. C.
Partington
P.
, and
Roberts
G. C. K.
, J. Chem. Phys. 60 , 2890 (1974 ).
23.
Wittebort
R. J.
and
Szabo
A.
, J. Chem. Phys. 69 , 1722 (1978 ).
24.
Connor
T. M.
, Trans. Faraday Soc. 60 , 1574 (1964 ).
25.
King
R.
and
Jardetzky
O.
, Chem. Phys. Lett. 55 , 15 (1978 ).
26.
King
R.
Maas
R.
Gassner
M.
Nanda
R. K.
Conover
W. W.
, and
Jardetzky
O.
, Biophys. J. 6 , 103 (1978 ).
27.
Wang
M. C.
, and
Uhlenbeck
G. E.
, Rev. Mod. Phys. 17 , 323 (1945 ).
28.
Kalk
A.
and
Berendsen
H. J. C.
, J. Mag. Res. 24 , 343 (1976 ).
29.
Richarz
R.
and
Wuthrich
K.
, Biochemistry 17 , 2263 (1978 ).
30.
Gelin
B. R.
and
Karplus
M.
, Proc. Natl. Acad. Sci. U.S.A. 72 , 2002 (1975 ).