An instrument for digital Raman difference spectroscopy which uses a programmed sample carrier for automated alternate positioning of reference and sample cells in the laser beam is described. Conventional single photon counting equipment is employed with its attendant advantages over dc analog methods. Signal processing is done with a digital computer. There are relatively few limitations on the types of sample cells which may be used. Applications to studies of dilute aqueous solutions of biological molecules, particularly, are discussed.
BodenheimerJ. S.BerenblutB. J., and WilkinsonG. R., Chem. Phys. Letters14, 533 (1972).
2.
KieferW., Appl. Spectrosc.27, 253 (1973).
3.
JonesR. N.BachT. E.FuhrerH.KarthaV. B.PithaJ.SeshadriK. S.VenkataraghavanR., and YoungR. P., National Research Council of Canada Bulletin No. 11 (1968).
4.
SavtizkyA. and GolayM. J. E., Anal. Chem.36, 1627 (1964).
5.
A deck listing of RSPC can be provided. Copies of RAMAN can be provided most conveniently if a reel of magnetic tape is sent to us. Only 7-track tapes can be produced.
6.
HildebrandJ. H.PrausnitzJ. M., and ScottR. L., Regular and Related Solutions (Van Nostrand Reinhold, New York, 1970), p. 184 and Appendix 5.
7.
MansyS.SprowlesJ. C.WoodT. E., and TobiasR. S., J. Am. Chem. Soc. in press.
8.
LordR. C. and ThomasG. J.Jr., Spectrochim. Acta23A, 2551 (1967).
