Transmission and attenuated total reflection (ATR) spectra of collagen and normal, adult human bone have been compared with literature data and band assignments for bone spectra are given. Except for slight shifts in some absorption bands (the amide I band at 1650 cm−1 and some of the phosphate absorption bands), most frequencies of both spectra are in fair agreement with previously reported values for the individual components. An improvement in resolution of some protein bands was achieved with the ATR technique.
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