A Technique for Obtaining the Ultraviolet Absorption Spectrum of Solid Keratin

Abstract

A technique is described for obtaining spectra of solid keratin in the form of thin sections of horse tail hair. The method of sectioning, mounting the specimen, and positioning it in the beam of the spectrophotometer is given. The application of a correction procedure for scattering, which his previously been described by other authors, and a corrected ultraviolet absorption spectrum of keratin are included in the report

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References

Speakman

J. B.

and McMahon

P. R.

, NEW ZEALAND J. SCI. TECHNOL. 22, 235B (1941)

Perutz

M. F.

Jope

, and Barer

, DISCUSSIONS FARADAY SOC. 9, 423 (1950)

Bendit

E. G.

, J. TEXTILE INST. 51, T544 (1960)

Thomson

, J. SCI. INSTR. 34, 333 (1957)

Goodwin

T. W.

and Morton

R. A.

, BIOCHEM. J. 40, 628 (1946)

Beaven

G. H.

and Holiday

E. R.

, ADVANCES IN PROTEIN CHEM. 7, 319 (1952)

Keilin

and Hartree

E. F.

, BIOCHIM. BIOPHYS. ACTA 27, 173 (1958)

Schramm

and Dannenberg

, BER. 77, 53 (1944)

Schauenstein

, MONATSH. CHEM. 80, 821 (1949)

10.

Schauenstein

, J. POLYMER. SCI. 16, 45 (1955)

11.

Treiber

, KOLLOID Z. 130, 39 (1953)

12.

Walker

P. M. B.

in General Cytochemical Methods, Danielli

J. F.

, Ed., Academic Press, N. Y., 1958, v. 1, p. 163

13.

Doty

and Edsall

J. T.

, ADVANCES IN PROTEIN CHEM. 6, 35 (1951)

14.

Heller

and Vassey

, PHYS. REV. 63, 65 (1943)

15.

Heller

and Vassey

, J. CHEM. PHYS. 14, 565 (1956)