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Abstract

Luminescence lifetimes are widely used as an analysis tool. Since decays in analytical systems are frequently complex decays rather than single exponentials, apparent lifetime methods based on the rapid lifetime determination (RLD) method or single frequency phase shift (SFPS) measurements are frequently used to reduce cost and simplify data analysis. It is demonstrated here that these methods can produce large errors under the right conditions. Both methods can give unexpected and uncharacteristic Stern-Volmer quenching plots (SVQPs) in two-component systems. Behaviors include bimodal quenching curves as well as “anti-quenching” curves. These phenomena are exacerbated by small fractions of long unquenched components.

Keywords

Stern–Volmer quenching Artifacts Anti-quenching Luminescence

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References

Lakowicz

J. R.

, “Emerging Biomedical Applications of Time-Resolved Fluorescence Spectroscopy”, in Topics in Fluorescence Spectroscopy, Lakowicz

J. R.

Ed. (Plenum Press, New York, 1994), vol. 4.

Engler

R. H.

Klein

and Trinks

, Meas. Sci. Technol. 11, 1077 (2000).

Clarke

Y. C.

Demas

J. N.

and DeGraff

B. A

, Anal. Chem. 72, 3468 (2000).

Carraway

E. R.

Demas

J. N.

and Bacon

J. R

, Anal. Chem. 63, 337 (1991).

Higgins

DeGraff

B. A.

and Demas

J. N

, Inorg. Chem. 44, 6662 (2005).

Takato

Gokan

and Daneko

, J. Photochem. Photobiol., A 169, 109 (2004).

Hupp

J. T.

Keefe

M. H.

and Benkstein

K. D

, Coord. Chem. Rev. 205, 201 (1999).

Rowe

, Ph.D. Thesis, University of Virgina, Charlottesville, VA (2004).

Bacon

J. R.

and Demas

J. N

, Anal. Chem. 59, 2780 (1987).

10.

J. L.

Schmehl

R. H.

and Xia

W. S

, Tetrahedron 56, 7045 (2000).

11.

Schäferling

Enderlein

Bauer

and Wolfbeis

O. S

, Appl. Spectrosc. 57, 1386 (2003).

12.

T. Q.

and Melton

L. A

, Appl. Spectrosc. 47, 773 (1993).

13.

Woods

R. J.

Scypinski

Love

L. J. C.

and Ashworth

H. A

, Anal. Chem. 56, 1395 (1984).

14.

Ballew

R. M.

and Demas

J. N

, Anal. Chem. 61, 30 (1989).

15.

Ballew

R. M.

and Demas

J. N

, Anal. Chim. Acta 245, 121 (1991).

16.

Sharaman

K. K.

Periasamy

Asheworth

Demas

J. N.

and Snow

N. H

, Anal. Chem. 71, 947 (1999).

17.

Chan

S. P.

Fuller

Z. J.

Demas

J. N.

and DeGraff

B. A

, Anal. Chem. 73, 4486 (2001).

18.

Koen

P. A.

Maliwal

B. P.

and Lakowicz

J. R

, US Patent 5,246,867 (1992).

19.

Tolosa

Szmacinski

Rao

and Lakowicz

J. R

, Anal. Biochem. 250, 102 (1997).

20.

Stepinac

T. K.

Chamot

S. R.

Rungger-Brändle

Ferrez

Munoz

Van den Bergh

Riva

C. E.

Pournaras

C. J.

and Wagnières

G. A

, Invest. Opthalmol. Vis. Sci. 46, 956 (2005).

21.

DeGraff

B. A.

and Demas

J. N

, “Luminescence-Based Oxygen Sensors”, Reviews in Fluorescence, Geddes

and Lakowicz

J. R

, Eds. (Springer Science, New York, 2005), vol. 2, pp. 125–151.

22.

Lin

C. T.

Boettcher

Chou

Creutz

and Sutin

N. J

, J. Am. Chem. Soc. 98, 6536 (1976).

23.

Lakowicz

J. R.

, Principles of Fluorescence Spectroscopy (Plenum Press, New York, 1983), 1st ed., p. 80.

24.

Buck

S. M.

Kopelman

Philbert

M. A.

Brasuel

Monson

Behrend

Ross

Rehemtulla

and Lee Koo

Y.-E

, “Fluorescent PEBBLE Nano-sensor and Nanoexplorers for Real-Time Intracellular and Biomedical Applications”, in Topics in Fluorescence Spectroscopy, Part B, Advanced Concepts in Fluorescence Spectroscopy. Macromolecular Sensing, Geddes

and Lakowicz

J. R

, Eds. (Spring Science and Business Media, New York, 2005), Vol. 10, Chap. 4, pp. 69–126.

25.

Jain

Demas

J. N.

and DeGraff

B. A

, Inorg. Chem. 37, 1876 (1998).

Abnormal Behavior in Stern—Volmer Luminescence Quenching Measurements via Apparent Lifetime Methods

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