Invasive candidiasis, defined as candidemia and disseminated candidiasis, is the most common fungal infection in hospitalized patients. In the current study, we used Fourier transform infrared (FT-IR) spectroscopy as a rapid, non-perturbing technique to investigate the effects of disseminated candidiasis on mouse liver tissues at the molecular level. The results revealed that the infection caused compositional changes in the tissues by decreasing the lipid content and the ratio of the saturated lipids to unsaturated lipids. An increase in the lipid/protein ratio was also observed. In addition, investigation of the olefinic band at 3014 cm−1 showed that lipid peroxidation took place in the infected samples. These results indicate that FT-IR spectroscopy is a promising technique for the evaluation and diagnosis of disseminated candidiasis.
Cheol-SangH.Gi-EunR.Seong-TaeK.Yeon-RanK.Won-KiH.Yong-UnB. and Sa-OukK, Biochim. Biophys. Acta1427, 245 (1999).
2.
BruneauJ. M.MailletI.TagatE.LegrandR.SupattoF.FudaliC.CaerJ. P.LabasV.LecaqueD. and HodgsonJ, Proteomics3, 325 (2003).
3.
ZaoutisT. E.GrevesH. M.LautenbachE.BilkerW. B. and CoffinS. E, Pediatr. Infect. Dis. J.23, 635 (2004).
4.
BretagneS. and CostaJ. M, FEMS Immunol. Med. Microbiol.45, 361 (2005).
5.
MaaroufiY.AharizN.HussonM. and CrokaertF, J. Clin. Microbiol.42, 3159 (2004).
6.
PitarchA.AbianJ.CarrascalM.SanchezM.NombelaC. and GilC, Proteomics4, 3084 (2004).
7.
SmithP. B.SteinbachW. J. and BenjaminD. K, Drug Resistance Updates8, 147 (2005).
8.
BendelC. M., Seminars in Perinatology27, 357 (2003).
9.
LeidichS. D.IbrahimA. S.FuY.KoulA.JessupC.VitulloJ.FonziW.MirbodF.NakashimaS.NozawaY. and GhannoumM. A, J. Biol. Chem.273, 26078 (1998).
10.
MorrisonC. J.HurstS. F. and ReissE, Clin. Diag. Lab. Immunol.10, 835 (2003).
11.
De RepentignyL., Curr. Opin. Microbiol.7, 324 (2004).
12.
RobozJ.NievesE. and HollandJ. F, J. Chromatogr.500, 413 (1990).
13.
RobozJ., Chirality6, 51 (1994).
14.
MasoodA. and SallahS, Leukemia Res.29, 493 (2005).
15.
SallahS.SemelkaR.KelekisN.WorawattanakulS. and SallahW, Acta Haematol.100, 77 (1998).
16.
SemelkaR. C.KelekisN. L.SallahS.WorawattanakulS. and AscherS. M, AJR Am. J. Roentgenol.169, 1311 (1997).
17.
SevercanF.ToyranN.KaptanN. and TuranB, Talanta53, 55 (2000).
18.
ToyranN.ZorluF.DonmezG.OgeK. and SevercanF, Eur. Biophys. J.33, 549 (2004).
19.
SevercanF.SahinI. and KazanciN, Biochim. Biophys. Acta, Biomembranes1668, 215 (2005).
20.
RomeoM. J.WoodB. R.QuinnM. A. and McNaughtonD, Biopolymers72, 69 (2003).
21.
RomeoM.MatthausC.MiljkovicM. and DiemM, Biopolymers74, 168 (2004).
22.
SevercanF.GorguluG.GorguluS. T. and GurayT, Anal. Biochem.339, 36 (2005).
23.
ToyranN.LaschP.NaumannD.TuranB. and SevercanF, Biochem. J.397, 427 (2006), BJ20060171.
24.
SevercanF.KaptanN. and TuranB, Spectrosc. Int. J.17, 569 (2003).
25.
LevinI. W. and BhargavaR, Annu. Rev. Phys. Chem.56, 429 (2005).
26.
LaschP.HaenschW.NaumannD. and DiemM, Biochim. Biophys. Acta1688, 176 (2004).
27.
MordehaiJ., J. Ramesh, M. Huleihel, Z. Cohen, O. Kleiner, M. Talyshinsky, V. Erukhimovitch, A. Cahana, A. Salman, R. K. Sahu, H. Guterman, and S. Mordechai, Biopolymers73, 494 (2004).
28.
ErukhimovitchV.TalyshinskyM.SouprunY. and HuleihelM, Methods Mol. Biol.292, 161 (2005).
29.
SchmidtA., “A Generalized Candida albicans Infection Model in the Rat”, in Handbook of Animal Models of Infection, ZakO. and SandeM. A, Eds. (Academic Press, New York, 1999), p. 649.
30.
GrellE., Membrane Spectroscopy (Springer-Verlag, Berlin, Heidelberg, 1981), p. 281.
31.
JaminN.DumasP.MoncuitJ.FridmanW.TeillaudJ.CarrL. G. and WilliamsG. P, Appl. Biol. Sci.95, 4837 (1998).
32.
LymanD. J. and Murray-WijelathJ, J. Biomed. Mater. Res.48, 172 (1999).
33.
DovbeshkoG. I.GridinaN. Y.KruglovaE. B. and PashchukO. P, Talanta53, 233 (2000).
34.
MelinA. M.PerromatA. and DelerisG, Biopolymers57, 160 (2000).
35.
CakmakG.ToganI. and SevercanF, Appl. Spectrosc.57, 835 (2003).
36.
TakahashiH.FrenchS. M. and WongP. T. T, Clin. Exp. Res.15, 219 (1991).
37.
WetzelD. L.SlatkinD. N. and LevineS. M, Cell. Mol. Biol.44, 15 (1998).
38.
JacksonM.RamjiawanB.HewkoM. and MantschH. H, Cell. Mol. Biol.44, 89 (1998).
39.
CutlerJ. E., Annu. Rev. Microbiol.45, 187 (1991).
40.
CotranR. S.RobbinsS. L. and KumarV, Robbins Pathologic Basis of Disease (W. B. Saunders Company, New York, 1994), 5th ed.
41.
RubinE. and FarberJ. L, Pathology (J. P. Lippincott Company, Philadelphia, 1988).
42.
KnolleP.LohrH.TreichelU.DienesH. P.LohseA.SchlaackJ. and GerkenG, Z. Gastroenterol.33, 613 (1995).
43.
DickoA.MorissetteM.AmeurS. B.PezoletM. and PaoloT, Brain Res. Bull.49, 401 (1999).
44.
LiuK.BoseR. and MantschH. H, Vib. Spectrosc.28, 131 (2002).
45.
SegalE. and EladD, “Candida species and Blastoschizomyces capitatus”, in Topley & Wilson'sMicrobiology and Microbial infections, Medical Mycology, AjelloL. and HayR. J, Eds. (Oxford University Press, New York, 1998), 9th ed., Vol. 4, p. 423.
