Abstract
Liquid core optical fiber (LCOF) waveguides have been used to enhance the Raman sensitivity of liquid sample measurements by more than two orders of magnitude. These waveguides can also enhance photobleaching of fluorescence in transparent liquids. We report the reduction of fluorescence intensity by more than two orders of magnitude by rapid photobleaching of solutions illuminated in an LCOF. The same solutions showed no detectable photobleaching when illuminated in a cuvette. The observed rates of photobleaching are in good quantitative agreement with theory.
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References
1.
Mackenzie
S. J.
and
Dakin
J. P
, Technical Digest, Conference Lasers and Electro-Optics Europe , 206 (1996 ).
2.
Li
J. Z.
Dasgupta
P. K.
and
Genfa
Z
, Talanta 50 , 617 (1999 ).
3.
Lacki
P.
Nowakowski
A.
Dress
P.
and
Franke
H
, Proc. SPIE-Int. Soc. Opt. Eng. 3730 , 112 (1999 ).
4.
Altkorn
R.
Koev
I.
Van Duyne
R. P.
and
Litorja
M
, Appl. Opt. 36 , 8992 (1997 ).
5.
Song
Li.
Liu
S.
Zhelyaskov
V.
and
El-Sayed
M. A
, Appl. Spectrosc. 52 , 1364 (1998 ).
6.
Holtz
M.
Dasgupta
P. K.
and
Zhang
G
, Anal. Chem. 71 , 2934 (1999 ).
7.
Altkorn
R.
Koev
I.
and
Pelletier
M. J
, Appl. Spectrosc. 53 , 1169 (1999 ).
8.
Dijkstra
R. J.
Bader
A. N.
Hoornweg
G.
Brinkman
U. A.
and
Gooijer
C
, Anal. Chem. 71 , 4575 (1999 ).
9.
Marquardt
B. J.
Vahey
P. G.
Synovec
R. E.
and
Burgess
L. W
, Anal. Chem. 71 , 4808 (1999 ).
10.
Waterbury
R. D.
Yao
W.
and
Byrne
R. H
, Anal. Chim. Acta 357 , 99 (1997 ).
11.
Yao
W. S.
Byrne
R. H.
and
Waterbury
R. D
, Environ. Sci. Technol. 32 , 22646 (1998 ).
12.
Dasgupta
P. K.
Genfa
Z.
Poruthoor
S. K.
Caldwell
S.
Dong
S.
and
Liu
S.-Y
, Anal. Chem. 70 , 4661 (1998 ).
13.
Yao
W. S.
and
Byrne
R. H
, Talanta 48 , 277 (1999 ).
14.
Dasgupta
P. K.
Genfa
Z.
Li
J.
Boring
C. B.
Jambunathan
S.
and
Al-Horr
R
, Anal. Chem. 71 , 1400 (1999 ).
15.
Lowry
J. H.
Mendlowitz
J. S.
and
Subramanian
N. S
, Opt. Eng. 31 , 1982 (1992 ).
16.
Pinnau
I.
and
Toy
L. G
, J. Membr. Sci. 109 , 125 (1996 ).
17.
Hamaguchi
H.
Tahara
T.
and
Tasumi
M
, Appl. Spectrosc. 41 , 1265 (1987 ).
18.
Affleck
R. L.
Ambrose
W. P.
Demas
J. N.
Goodwin
P. M.
Schecker
J. A.
Wu
M.
and
Keller
R. A
, Anal. Chem. 68 , 2270 (1996 ).
19.
Pelletier
M. J.
and
Altkorn
R
, Anal. Chem. , paper submitted.
20.
Owen
H.
Battey
D. E.
Pelletier
M. J.
and
Slater
J. B
, Proc. SPIE-Int. Soc. Opt. Eng. 2406 , 260 (1995 ).
21.
Owen
H.
Tedesco
J. M.
and
Slater
J. B
, U. S. Patent 5,377,004 (1994 ).
22.
Battey
D. E.
Slater
J. B.
Wludyka
R.
Owen
H.
Pallister
D. M.
and
Morris
M. D
, Appl. Spectrosc. 47 , 1913 (1993 ).
23.
Battey
D. E.
Owen
H.
and
Tedesco
J. M
, U.S. Patent 5,559,597 (1996 ).
24.
Eggeling
C.
Widengren
J.
Rigler
R.
and
Seidel
C. A. M
, Anal. Chem. 70 , 2651 (1998 ).
25.
Goodwin
P. M.
Ambrose
W. P.
and
Keller
R. A
, Acc. Chem. Res. 29 , 607 (1996 ).
26.
Soper
S. A.
Nutter
H. L.
Keller
R. A.
Davis
L. M.
and
Shera
E. B
, Photochem. Photobiol. 57 , 972 (1993 ).
27.
Brackmann
U.
, Lambdachrome Laser Dyes (Lambda Physik GmbH , Gottingen, Germany , 1986 ), pp. 3 –117 .
28.
Wang
W.
He
Q.
Wang
T.
Fen
M.
Liao
Y.
and
Ran
G
, Anal. Chem. 64 , 22 (1991 ).
29.
He
Q. S.
Wang
W.
Wu
G. Y.
Wang
J. F.
Fen
M. Z.
He
T. A.
Ly
Pan L. Y.
and
Zhao
Y. S
, Spectrosc. Spectral Anal. 19 , 95 (1999 ).
30.
Belz
M.
Dress
P.
Sukhitskiy
A.
and
Liu
S
, Proc. SPIE-Int. Soc. Opt. Eng. 3856 , 271 (1999 ).
31.
Song
L.
Varma
C. A. G. O.
Verhoeven
J. W.
and
Tanke
H. J
, Biophys. J. 70 , 2959 (1996 ).