Abstract
The authors describe the use of an ultraviolet light-emitting diode (UV LED), Nichia NSHU590E, as a 373 nm excitation light source for fluorescence lifetime measurements. A frequency-domain technique has been chosen to demonstrate that the UV LED can be efficiently intensity modulated by driving the radio-frequency signal over a range of modulation frequencies from 10 kHz up to 330 MHz. Time-resolved measurements have been performed by using a fluorophore with a very long lifetime of 10.2 μs and fluorophore with a very short lifetime of 0.15 ns. Application of LEDs for lifetime-based chemical sensing is discussed.
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References
1.Topics in Fluorescence Spectroscopy ,
Lakowicz
J. R.
, Ed. (Plenum Press , New York , Vols. 1–2 , 1991 , Vol. 3 , 1993 , Vol. 4 , 1994 ).
2.
Szmacinski
H.
and
Lakowicz
J. R
, in Topics in Fluorescence Spectroscopy ,
Lakowicz
J. R.
, Ed. (Plenum Press , New York , 1994 ), Vol. 4 , pp. 295 –334 .
3.
Sipior
J.
Carter
G. M.
Lakowicz
J. R.
and
Rao
G
, Rev. Sci. Instrum. 67 , 3795 , (1996 ).
4.
Thompson
R. B.
Frisoli
J. K.
and
Lakowicz
J. R
, Anal. Chem. 64 , 2075 (1992 ).
5.
Berndt
K. W.
Gryczynski
I.
and
Lakowicz
J. R
, Rev. Sci. Instrum. 61 , 1816 (1990 ).
6.
Kalyanasundaram
K.
and
Grätzel
M
, Coordination Chem. Rev. 77 , 347 (1998 ).
7.
Shen
Y.
and
Sullivan
B. P
, Chem. Educ. 74 , 685 (1997 ).
8.
Lakowicz
J. R.
Terpetschnig
E.
Murtaza
Z.
and
Szmacinski
H
, J. Fluoresc. 7 , 17 (1997 ).
9.
Lakowicz
J. R.
Murtaza
Z.
Jones
W. E.
Jr.
Kim
K.
and
Szmacinski
H
, J. Fluoresc. 6 , 245 (1996 ).
10.
Szmacinski
H.
and
Lakowicz
J. R
, Sens. Actuators , (1999 ), paper in press.
11.
Laczko
G.
Gryczynski
I.
Gryczynski
Z.
Wiczk
W.
Malak
H.
and
Lakowicz
J. R
, Rev. Sci. Instrum. 61 , 2331 (1990 ).
12.
Gruber
W. R.
O'Leary
P.
and
Wolfbeis
O
, Proc. SPIE-Int. Soc. Opt. Eng. 2137 , 190 (1994 ).
13.
O'Keeffe
G.
MacCraith
B. D.
McEvoy
A. K.
McDonagh
C. M.
and
McGilp
J. F
, Sens. Actuators, B 29 , 226 (1995 ).
14.
Harms
P.
Sipior
J.
Ram
N.
Carter
G. M.
and
Rao
G
, Rev. Sci. Instrum. 70 , 1535 (1999 ).