Abstract
Morel, G., and Cavalier, A. (eds.). In Situ Hybridization in Light Microscopy, 1st ed. 327 pp. CRC Press LLC, Boca Raton, FL, 2001. $119.95. ISBN 0-8493-0703-1.
The objective of this book is to provide theoretic and technical information on the different stages of in situ hybridization by light microscopy. Each of the first six chapters focuses on a specific stage of the method: probes, tissue preparation, pretreatments, hybridization, revelation of hybrids, and counterstaining and modes of observation. The chapters offer a complete list of alternatives available, with comments on their advantages and disadvantages. Chapter 7 addresses issues related to background, sensitivity/specificity, and controls. Chapter 8 provides several reference protocols with helpful technical tips and comments regarding potential adjustments. Finally, the authors illustrate the expected results using selected examples consisting of color photomicrographs and technical comments. Two appendices listing materials, solutions, and reagents; a comprehensive list of abbreviations; and a glossary are also available. The index is user friendly, and each chapter starts with a very detailed table of contents.
The authors use an original two-column layout that separates the theory from the practical aspects. Because of the breadth of information included in this book, the authors use a very well-organized format with numbering, bullet points, bolding, different-colored backgrounds, and short sentences that allow the reader to quickly locate the information needed. There are numerous conceptual schematics that illustrate very well the theoretic aspect of the most complex techniques. In addition, when necessary, tables provide a rapid summary of the advantages and disadvantages of the alternatives and help the reader choose the most appropriate procedure.
The book is designed to be a daily reference for the histology or molecular pathology laboratory and is intended for laboratory students, new or experienced histologists, and seasoned researchers. Because of its completeness, format, and practical aspect, it is definitely a valuable addition for any laboratory routinely performing or trying to develop in situ hybridization. In addition, it is a very useful and unique technical reference for the veterinary pathologist.