Stability of Cisplatin,Doxorubicin,and Mitomycin Combined with Ioversol for Chemoembolization

Abstract

OBJECTIVE:

To evaluate the chemical stability of a mixture of cisplatin 10 mg/mL, doxorubicin 5 mg/mL, and mitomycin 1 mg/mL in sodium chloride 0.9% (NS), and sodium chloride 0.9% and ioversol 68% (NSI).

METHODS:

The agents were reconstituted with NS and NSI in two groups. One syringe was made and stored protected from light at 4, 25, and 37 °C. Triplicate HPLC determinations were performed on each syringe at each time period. Drug concentration at T₀, 4, 12, 24, 72, and 120 hours were recorded.

RESULTS:

Cisplatin in NS remained stable for five days at all three temperatures and lost 12.7% at 37 °C in 120 hours in NSI. Doxorubicin in NS remained stable throughout the five days at 4 and 25 °C, but lost 13.1% at 37 °C in 24 hours. Doxorubicin in NSI remained stable for five days at 4 °C and lost 16.3% at 25 °C by day 5, and 18.8% at 37 °C in 24 hours. Mitomycin in NS lost 15.8% at 4 °C within 24 hours, and 24.7% at 25 °C and 36% at 37 °C within four hours. Mitomycin in NSI was stable for 72 hours at 4 °C, 24 hours at 25 °C, and less than four hours at 37 °C.

CONCLUSIONS:

These agents in NS are stable for 12 hours at 4 °C. In NSI, they are stable for 72 hours at 4 °C.