We report the use of hypertonic saline and sodium hydroxide (HS-SH) for concentration-decontamination of sputum samples for the diagnosis of tuberculosis. This method increases detection of mycobacteria in smears compared to examination of direct smears, is comparable and reduces contamination in cultures compared to NALC-NaOH.
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References
1.
SuárezP, WattC, AlarcónE, The dynamics of tuberculosis in response to 10 years of intensive control effort in Perú. J Infect Dis2001;184:473–8
2.
AberVR, AllenBW, MitchisonDA, AyumaP, EdwardsEA, KeyesAB. Quality control in tuberculosis bacteriology. Laboratory studies on isolated positive cultures and the efficiency of direct smear examination. Tubercle1980;61:123–33
3.
AngebyKAK, Alvarado-GálvezC, Pineda-GarcíaL, HoffnerE. Improved sputum microscopy for a more sensitive diagnosis of pulmonary tuberculosis. Int J Tuber Lung Dis2000;7:684–7
4.
KingM, DasguptaB, TomkiewiczRP, BrownNE. Rheology of cystic fibrosis sputum after in vitro treatment with hypertonic saline alone and in combination with recombinant human deoxyribonuclease. Am J Resp Crit Care Med1997;156:173–7
5.
KentBD, KubicaGP. Public Health Mycobacteriology: a Guide for the Level II Laboratory. Atlanta, U.S. Department of Health and Human, 1985
