Autologous mesenchymal stem cell (MSC) transplants have been used successfully to treat a number of diseases, and patients undergoing cell transplantation must have stem cells collected before transplantation. In this study, we developed a new method to harvest MSCs. Biomaterials were implanted into the spatium intermuscular of mice hind limbs, and a large number of migrating cells (MCs) were isolated from the transplanted biomaterials. The adherent cells in MCs showed the characteristics of MSCs. Further comparative study demonstrated that the characteristics of MC-MSCs were similar to that of bone marrow (BM)-MSCs, including morphology, phenotype, proliferation potential, multilineage differentiation capacity and hematopoiesis-supportive function. The colony-forming unit-fibroblast frequency of the MCs was equivalent to approximately 20-fold of that of the BM. In addition, a BM transplantation experiment demonstrated that MC-MSCs were derived from the peripheral blood. In conclusion, we successfully establish an efficient method to harvest MSCs, and together with the distinct advantages of this method, such as accessibility and possibility for autologous cell therapy, we conclude that our efficient method may be a promising alternative for clinical application.
NejadnikH, HuiJH, ChoongEP, TaiBC, LeeEH. Autologous bone marrow-derived mesenchymal stem cells versus autologous chondrocyte implantation: an observational cohort study. Am J Sports Med2010;38:1110–6
2.
KramperaM, PizzoloG, ApriliG, FranchiniM. Mesenchymal stem cells for bone, cartilage, tendon and skeletal muscle repair. Bone2006;39:678–83
3.
KassemM, AbdallahBM. Human bone-marrow-derived mesenchymal stem cells: biological characteristics and potential role in therapy of degenerative diseases. Cell Tissue Res2008;331:157–63
4.
SalemHK, ThiemermannC. Mesenchymal stromal cells: current understanding and clinical status. Stem Cells2010;28:585–96
5.
ElvenesJ, KnutsenG, JohansenO, MoeBT, MartinezI. Development of a new method to harvest chondroprogenitor cells from underneath cartilage defects in the knees. J Orthop Sci2009;14:410–7
6.
PengL, JiaZ, YinX, ZhangX, LiuY, ChenP, MaK, ZhouC. Comparative analysis of mesenchymal stem cells from bone marrow, cartilage, and adipose tissue. Stem Cells Dev2008;17:761–73
7.
VieiraN, BrandaliseV, ZucconiE, SeccoM, StraussB, ZatzM. Isolation, characterization and differentiation potential of canine adipose-derived stem cells. Cell Transplant2009;19:279–89
8.
WuX, WangS, ChenB, AnX. Muscle-derived stem cells: isolation, characterization, differentiation, and application in cell and gene therapy. Cell Tissue Res2010;340:549–67
9.
HuangJI, KazmiN, DurbhakulaMM, HeringTM, YooJU, JohnstoneB. Chondrogenic potential of progenitor cells derived from human bone marrow and adipose tissue: a patient-matched comparison. J Orthop Res2005;23:1383–9
10.
LiuW, CaoY. Application of scaffold materials in tissue reconstruction in immunocompetent mammals: our experience and future requirements. Biomaterials2007;28:5078–86
11.
WatanabeM, JoJ, RaduA, KanekoM, TabataY, FlakeAW. A tissue engineering approach for prenatal closure of myelomeningocele with gelatin sponges incorporating basic fibroblast growth factor. Tissue Eng Part A2010;16:1645–55
12.
RohanizadehR, SwainMV, MasonRS. Gelatin sponges (Gelfoam) as a scaffold for osteoblasts. J Mater Sci Mater Med2008;19:1173–82
13.
TakahashiY, YamamotoM, TabataY. Osteogenic differentiation of mesenchymal stem cells in biodegradable sponges composed of gelatin and beta-tricalcium phosphate. Biomaterials2005;26:3587–96
VerfaillieCM. Multipotent adult progenitor cells: an update. Novartis Found Symp2005;265:55–61; discussion–5, 92–7
16.
KallestadKM, McLoonLK. Defining the heterogeneity of skeletal muscle-derived side and main population cells isolated immediately ex vivo. J Cell Physiol. 2010;222:676–84
17.
AsaharaT, MuroharaT, SullivanA, SilverM, van der ZeeR, LiT, WitzenbichlerB, SchattemanG, IsnerJM. Isolation of putative progenitor endothelial cells for angiogenesis. Science1997;275:964–7
18.
HowellJC, LeeWH, MorrisonP, ZhongJ, YoderMC, SrourEF. Pluripotent stem cells identified in multiple murine tissues. Ann N Y Acad Sci2003;996:158–73
19.
McKinney-FreemanSL, JacksonKA, CamargoFD, FerrariG, MavilioF, GoodellMA. Muscle-derived hematopoietic stem cells are hematopoietic in origin. Proc Natl Acad Sci USA2002;99:1341–6
20.
TsuboiK, KawadaH, TohE, LeeYH, TsumaM, NakamuraY, SatoT, AndoK, MochidaJ, KatoS, HottaT. Potential and origin of the hematopoietic population in human skeletal muscle. Leuk Res2005;29:317–24
21.
CrisanM, YapS, CasteillaL, ChenCW, CorselliM, ParkTS, AndrioloG, SunB, ZhengB, ZhangL, NorotteC, TengPN, TraasJ, SchugarR, DeasyBM, BadylakS, BuhringHJ, GiacobinoJP, LazzariL, HuardJ, PeaultB. A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell2008;3:301–13
22.
PeaultB, RudnickiM, TorrenteY, CossuG, TremblayJP, PartridgeT, GussoniE, KunkelLM, HuardJ. Stem and progenitor cells in skeletal muscle development, maintenance, and therapy. Mol Ther2007;15:867–77
23.
LiJ, WangS, HanJ, YuS, ZhangC, ZhaoY. Cells captured from spatium intermusculare by porous material exhibit the characteristics of stem cells. Histochem Cell Biol2008;130:741–8
24.
YoshimuraH, MunetaT, NimuraA, YokoyamaA, KogaH, SekiyaI. Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle. Cell Tissue Res2007;327:449–62
25.
SunS, GuoZ, XiaoX, LiuB, LiuX, TangPH, MaoN. Isolation of mouse marrow mesenchymal progenitors by a novel and reliable method. Stem Cells2003;21:527–35
26.
SoleimaniM, NadriS. A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. Nat Protocol2009;4:102–6
27.
GharaibehB, LuA, TebbetsJ, ZhengB, FeduskaJ, CrisanM, PeaultB, CumminsJ, HuardJ. Isolation of a slowly adhering cell fraction containing stem cells from murine skeletal muscle by the preplate technique. Nat Protocol2008;3:1501–9
28.
CaoY, YaoZ, SarkarD, LawrenceM, SanchezGJ, ParkerMH, MacQuarrieKL, DavisonJ, MorganMT, RuzzoWL, GentlemanRC, TapscottSJ. Genome-wide MyoD binding in skeletal muscle cells: a potential for broad cellular reprogramming. Dev Cell2010;18:662–74
29.
PratherWR, TorenA, MeironM. Placental-derived and expanded mesenchymal stromal cells (PLX-I) to enhance the engraftment of hematopoietic stem cells derived from umbilical cord blood. Expert Opin Biol Ther2008;8:1241–50
30.
Le BlancK, SamuelssonH, GustafssonB, RembergerM, SundbergB, ArvidsonJ, LjungmanP, LonniesH, NavaS, RingdenO. Transplantation of mesenchymal stem cells to enhance engraftment of hematopoietic stem cells. Leukemia2007;21:1733–8
31.
ChapelA, BerthoJM, BensidhoumM, FouillardL, YoungRG, FrickJ, DemarquayC, CuvelierF, MathieuE, TrompierF, DudoignonN, GermainC, MazurierC, AigueperseJ, BornemanJ, GorinNC, GourmelonP, ThierryD. Mesenchymal stem cells home to injured tissues when co-infused with hematopoietic cells to treat a radiation-induced multi-organ failure syndrome. J Gene Med2003;5:1028–38
