The stability between two HIV-1 RNA measurements one year apart and the relationship with HIV subtype in rural Uganda

Abstract

We compared HIV-1 RNA levels using the nucleic acid sequenced based amplification (NASBA) test kit in 2 samples taken one year apart from participants infected with env subtype A or D in a population-based cohort in Uganda. Fifty participants were infected with subtype A and 70 with subtype D. HIV-1 RNA levels were significantly higher in subtype D unadjusted (P = 0.001), and after adjusting for age, gender, and CD4 count (P < 0.001). Eighty-six participants had HIV-1 RNA measurements in both years and 67 (78%) were within one log₁₀ of their result a year before. There was no relationship between the difference in log viral load and proportion of CD4 change. Individuals infected with subtype D had a higher average increase in viral load and this was statistically significant if adjusted for baseline levels and CD4 count (P = 0.015).