Abstract
38 gnotobiotic lambs have been derived by hysterectomy and reared for up to 7 months as true ruminants. 10 cases of contamination have occurred, 4 of which were attributable to a breach in the protective barrier and 1 to a contaminated food pack.
The technique permits the use of liquid and solid foods and the regular taking of rumen and other samples, and also regular removal of all excreta and the cleaning of lambs and cages.