A method for the rapid quantification of rosmarinic acid (RA) in tissues of spearmint using near infrared (NIR) spectroscopy was developed by correlating with the results of methanol extracts analysed on an HPLC photo-diode array (PDA) system. NIR and HPLC analyses performed on over 500 samples were used to develop an NIR analytical algorithm. Applying this algorithm resulted in very good calibration statistics for the prediction of RA concentrations in dried powdered samples prepared from the cultured mints (r2 = 0.90, SEP=3.1 RPD = 2.6). NIR can be used to rapidly and accurately quantitate the levels of this secondary metabolite in plant samples. High positive correlations occur between CO2 levels and spearmint plantlet growth (fresh weight), morphogenetic responses (leaves, roots and shoots) and production of the secondary metabolite rosmarinic acid.
PetersenM.S., “Characterization of rosmarinic acid synthase from cell cultures of Coleus blumei”, Phytochemistry30, 2877 (1991). doi: 10.1016/S0031-9422(00)98217-7
2.
ShettyK., “Biosynthesis and medical applications of rosmarinic acid,”J. Herbs Spices Med. Plants8, 161 (2001). doi: 10.1300/J044v08n0205
3.
YangR.ShettyK., “Stimulation of rosmarinic acid in shoot cultures of oregano (Origanum vulgare) clonal line in response to proline, proline analog and proline precursors”, J. Agric. Food Chem.46, 2888 (1998). doi: 10.1021/jf9802629
4.
ZenkM.El-ShagiH.UlbrichB., “Production of rosmarinic acid by cell suspension cultures of Coleus blumei”, Naturwissenschaffen64, 585 (1977). doi: 10.1007/BF00450645
5.
De-EknamkulW.EllisB., “Rosmarinic acid production and growth characteristics of Anchusa officinalis cell suspension cultures”, Planta Medica51, 346 (1984). doi: 10.1055/s-2007-969728
6.
WhitakerR.J.HashimotoT.EvansD.A., “Production of the secondary metabolite, rosmarinic acid, by plant cell suspension cultures”, Ann. NY Acad. Sci.435, 364 (1984). doi: 10.1111/j.1749-6632.1984.tb13822.x
7.
EllisB., “Characterization of clonal cultures of Anchusa officinalis derived from single cells of known productivity”, J. Plant Physiol.19, 149 (1985).
8.
KimH.K.OhS.-R.LeeH.-K.HuhH., “Benzothiadiazole enhances the elicitation of rosmarinic acid production in a suspension culture of Agastache rugosa O. Kuntze”, Biotech. Lett.23, 55 (2001). doi: 10.1023/A:1026738409671
9.
HippolyteI.MarinB.BaccouJ.C.JonardR., “Growth and rosmarinic acid production in cell suspension cultures of Salvia officinalis L.”, Plant Cell Rep.11, 109 (1992). doi: 10.1007/BF00232160
10.
ShettyK., “Biotechnology to harness the benefits of dietary phenolics; focus in Lamiaceae”, Asia Pacific J. Clin. Nutr.6, 162 (1997).
11.
KaramN.S.JawadF.M.ArikatN.A.ShibliR.A., “Growth and rosmarinic acid accumulation in callus, cell suspension, and root cultures of wild Salvia fruticosa”, Plant Cell Tiss. Org. Cult.73, 117 (2003). doi: 10.1023/A:1022806420209
12.
LiW.KoikeK.AsadaY.YoshikawaT.NikaidoT., “Rosmarinic acid production of Coleus forskohlii hairy root cultures”, Plant Cell Tiss. Org. Cult.80, 151 (2005). doi: 10.1007/s11240-004-9541-x
13.
SumaryonoW.ProkschP.HartmannT.NimtzM.WrayV., “Induction of rosmarinic acid accumulation in cell suspension cultures of Orthosiphon aristatus after treatment with yeast extract”, Phytochemistry30, 3267 (1991). doi: 10.1016/0031-9422(91)83190-V
14.
TisseratB.VaughnS.F., “Essential oils enhanced by ultra-high carbon dioxide levels from Lamiaceae species grown in vitro and in vivo”, Plant Cell Rep.20, 361 (2001). doi: 10.1007/s002990100327
15.
TisseratB.VaughnS.F.SilmanR., “Influence of modified oxygen and carbon dioxide atmospheres on mint and thyme plant growth, morphogenesis and secondary metabolism in vitro”, Plant Cell Rep.20, 912 (2002). doi: 10.1007/s00299-001-0428-6
16.
MitraA.KhanB.M.RawalS.K., “Photoautotrophic shoot culture: An economical alternative for the production of total alkaloid from Cartharanthus roseus (L.) G. Don”, Curr. Sci.73, 608 (1997).
17.
NogueiraJ.M.F.RomanoA., “Essential oils from micropropagated plants of Lavandula viridis”, Photochem. Anal.13, 4 (2002). doi: 10.1002/pca.609
18.
TisseratB.BerhowM.A.VaughnS.F., “Elevated carbon dioxide levels enhance rosmarinic acid production in spearmint plantlets”, manuscript submitted to Plant Cell Organ Tiss. Cult. (2007).
ShultzH.DrewsH.-H.QuilitzschR.KruegerH., “Application of near infrared spectroscopy for the quantification of quality parameters in selected vegetables and essential oil plants”, J. Near Infrared Spectrosc.6, A125 (1998).
21.
WilliamsP.C.NorrisK.H., “Qualitative applications of near-infrared reflectance spectroscopy”, in: Near infrared technology in the agriculture and food industries, Ed by WilliamsP.NorrisK., American Association of Cereal Chemists, Inc., St Paul, MN, USA, pp. 23, 38 and 241 (1987).
22.
BattenG.D., “Plant analysis using near infrared reflectance spectroscopy: The potential and the limitations”, Austral. J. Exper. Agric.38, 697 (1998). doi: 10.1071/EA97146
23.
SchulzH.EngelhardtU.H.WegentA.DrewsH.-H.LapczynskiS., “Application of near-infrared reflectance spectroscopy to the simultaneous prediction of alkaloids and phenolic substances in green tea leaves”, J. Agric. Food Chem.47, 5064 (1999). doi: 10.1021/jf9813743
24.
GrayD.E.RobertsC.A.RottinghausG.E.GarnettH.E.PallardyS.G., “Quantification of root chicoric acid in purple coneflower by near infrared reflectance spectroscopy”, Crop Sci.41, 1159 (2001).
25.
SchulzH.SteuerB.KrugerH.SchulzeW.JunghannsW.WeinreichB., “Schnelle erfassung von qualitätsparametern in rosmarinblättern (Rosmarinus officinalis L.) mittels nah-infrarotspektroskopie”, Z. Arzn. Gew. Pfl.6, 79–84 (2001).
26.
SteuerB.ShulzH.KrugerH., “Fast determination of minor components in rosemary leaves by near infrared spectroscopy”, in Near infrared spectroscopy: Proceedings of the 9th international conference, Ed by DaviesA.M.C.GiangiacomoR., NIR Publications, Chichester, West Sussex, UK, pp. 499–501 (2000).
27.
ShulzH.QuilitzschR., “Determination of active ingredients in vegetable drugs using diamond ATR-IR spectroscopy”, GIT Lab. J. Eur.7, 86–88 (2003).
28.
ShulzH.SteuerB.PfefferS.KrugerH., “Potential of near infrared spectroscopy for supporting breeding and cultivation of medicinal and spice plants”, NIR news13, 10–11 (2002).
29.
QuilitzschR.ShulzH., “Quantitative bestimmungen an kamillenölen, sonnenhutwurzeln und rosmarinblättern mit einem portablen ATR-IR-spektrometer”, Z. Arzn. Gew. Pfl.7, Sonderausg. 85–90 (2002).
30.
SchultzH.DrewsH.-H.KruegerH., “Rapid NIRS determination of quality parameters in leaves and isolated essential oils of Mentha species,”J. Essent. Oil Res.11, 185 (1999).
31.
StehfestK.BoeseM.KernsG.PiryA.WilhelmC., “Fourier transform infrared spectroscopy as a new tool to determine rosmarinic acid in situ”, J. Plant Physiol.161, 151 (2004). doi: 10.1078/0176-1617-01099
32.
MurashigiT.SkoogF., “A revised medium for rapid growth and bioassays with tobacco tissue cultures”, Physiol. Plant.15, 473 (1962). doi: 10.1111/j.1399-3054.1962.tb08052.x
33.
TisseratB.SilmanR., “Interactions of culture vessels, media volume, culture density, and carbon dioxide levels on lettuce and spearmint shoot growth in vitro”, Plant Cell Rep.19, 464 (2000). doi: 10.1007/s002990050757
WilliamsP.C., “Implementation of near-infrared technology”, in Near-infrared spectroscopy in the agricultural and food industries, 2nd Edition, Ed by WilliamsP.NorrisK., American Association of Cereal Chemists, St Paul, MN, USA, pp. 145–169 (2004).
