A method to isolate isomers in a Fourier transform ion cyclotron resonance mass spectrometer on the basis of their different hydrogen/deuterium exchange rates has been developed and applied to the protonated serine octamer cluster. Isolation allows interrogation of each isomer by infrared multi-photon dissociation to determine any differences in the building blocks that form these clusters. The experimental results strongly support previous assignments of an all-zwitterion structure to the slower-exchanging isomer and an all-neutral structure to its faster-exchanging counterpart.
CooksR.G.ZhangD.KochK.J.GozzoF.C.EberlinM.N., “Chiroselective Self-Directed Octamerization of Serine: Implications for Homochirogenesis”, Anal. Chem.73, 3646 (2001).
2.
JulianR.R.MyungS.ClemmerD.E., “Spontaneous Anti-Resolution in Heterochiral Clusters of Serine”, J. Am. Chem. Soc.126, 4110 (2004).
3.
JulianR.R.HodyssR.KinnearB.JarroldM.F.BeauchampJ.L., “Nanocrystalline Aggregation of Serine Detected by Electrospray Ionization Mass Spectrometry: Origin of the Stable Homochiral Gas-Phase Serine Octamer”, J. Phys. Chem. B106, 1219 (2002).
MazurekU.ReubenB.G.McFarlandM.A.MarshallA.G.LifshitzC., “Elucidating Structures of Protonated Amino Acid Clusters from H/D Exchange Experiments: The Protonated Serine Octamer”, 52nd ASMS Conference on Mass Spectrometry, Nashville, TN, USA (2004).
6.
SenkoM.W.HendricksonC.L.PasaTolicL.MartoJ.A.WhiteF.M.GuanS.MarshallA.G., “Electrospray Ionization Fourier Transform Ion Cyclotron Resonance at 9.4 T”, Rapid Commun. Mass Spectrom.10, 1824 (1996).
7.
ChowdhuryS.K.KattaV.ChaitB.T., “An electrospray-ionization mass spectrometer with new features”, Rapid Commun. Mass Spectrom.4, 81 (1990).
8.
HendricksonC.L.QuinnJ.P.EmmettM.R.MarshallA.G., “Mass-Selective External Ion Accumulation for Fourier Transform Ion Cyclotron Resonance Mass Spectrometry”, 49th ASMS Conference on Mass Spectrometry and Allied Topics, Chicago, IL, USA (2001).
9.
SenkoM.W.CanterburyJ.D.GuanS.MarshallA.G., “A High-Performance Modular Data System for Fourier Transform Ion Cyclotron Resonance Mass Spectrometry”, Rapid Commun. Mass Spectrom.10, 1839 (1996).
10.
JiaoC.Q.RanatungaD.R.A.VaughnW.E.FreiserB.S., “A Pulsed-Leak Valve for Use with Ion Trapping Mass Spectrometers”, J. Am. Soc. Mass Spectrom.7, 118 (1996).
11.
MarshallA.G.WangT.-C.L.RiccaT.L., “Tailored Excitation for Fourier Transform Ion Cyclotron Resonance Mass Spectrometry”, J. Am. Chem. Soc.107, 7893 (1985).
12.
SchalleyC.A.WeisP., “Unusually stable magic number clusters of serine with a surprising preference for homochirality”, Int. J. Mass Spectrom.221, 9 (2002).
13.
CountermanA.E.ClemmerD.E., “Magic Number Clusters of Serine in the Gas Phase”, J. Phys. Chem. B105, 8092 (2001).
