Abstract
A sensitive and selective high-performance liquid chromatography/atmospheric pressure chemical ionisation tandem mass spectrometry (HPLC/APCI-MS/MS) method for the simultaneous detection of 18 benzodiazepines and metabolites in human blood is described. The procedure utilises butyl chloride extraction at alkaline pH followed by reversed-phase liquid chromatography. The technique is suitable for screening analyses and confirmation of identity of the benzodiazepines at their lowest reported therapeutic concentrations using 500 μL of blood. The method has been successfully applied in forensic cases involving low concentrations of benzodiazepines.
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References
1.
Drummer
O.
, Forensic Sci. Rev. 14 , 1 (2002 ).
2.
Druid
H.
Holmgren
P.
and
Ahlner
J.
, Forensic Sci. Int. 122 , 136 (2001 ).
3.
Gaillard
Y.
Gay-Montchamp
J.
and
Ollagnier
M.
, J. Chromatogr. 622 , 197 (1993 ).
4.
Edeki
T.
Robin
D.
Prakash
C.
and
Blair
I.
, J. Chromatogr. 577 , 190 (1992 ).
5.
LeGatt
F.
and
McIntosh
D.
, Clin. Biochem. 26 , 159 (1993 ).
6.
Negrusz
A.
Moore
C.M.
Hinkel
K.B.
Stockham
T.L.
Verma
M.
Strong
M.J.
and
Janicak
P.G.
, J. Forensic Sci. 46 , 1143 (2001 ).
7.
Black
D.
Clark
G.
Haver
V.
Garbin
J.
and
Saxon
A.
, J. Anal. Toxicol. 18 , 185 (1994 ).
8.
Elsohly
M.
Feng
S.
Salomone
S.
and
Brenneisen
R.
, J. Anal. Toxicol. 23 , 486 (1999 ).
9.
Pirnay
S.
Ricordel
I.
Libong
D.
and
Bouchonnet
S.
, J. Chromatogr. A 954 , 235 (2002 ).
10.
Joyce
J.
Bal
T.
Ardrey
E.
Stevens
H.
and
Moffat
A.
, Biomed. Mass Spectrom. 11 , 284 (1984 ).
11.
McIntyre
I.
Syrjanen
M.
Crump
K.
Horomidis
S.
Peace
A.
and
Drummer
O.
, J. Anal. Toxicol. 17 , 202 (1993 ).
12.
Crouch
D.
Rollins
D.
Canfield
D.
Andrenyak
D.
and
Schulties
E.
, J. Anal. Toxicol. 23 , 479 (1999 ).
13.
Miki
A.
Tatsuno
M.
Katagi
M.
Nishikawa
M.
and
Tsuchihashi
H.
, J. Anal. Toxicol. 26 , 87 (2002 ).
14.
Marquet
P.
Baudin
O.
Gaulier
J.
Lacassie
E.
Dupuy
J.
Francois
B.
and
Lachatre
G.
, J. Chromatogr. B 734 , 137 (1999 ).
15.
Goeringer
K.E.
McIntyre
I.
and
Drummer
O.H.
, J. Anal. Toxicol. 27 , 30 (2003 ).
16.
Temesi
D.
and
Law
B.
, LC-GC 17 , 626 (1999 ).
17.
Baselt
R.C.
, Disposition of Toxic Drugs and Chemicals in Man. Biomedical Publications , Foster City, USA (2002 ).
18.
Weng
N.
Chen
Y.
Shou
W.
and
Jiang
X.
, J. Pharm. Biomed. Anal. 26 , 753 (2001 ).
19.
McCarley
T.D.
and
Brodbelt
J.
, Anal. Chem. 65 , 2380 (1993 ).
20.
McClean
S.
O'Kane
E.J.
and
Smyth
W.F.
, Electrophoresis 21 , 1381 (2000 ).
21.
Smyth
W.F.
McClean
S.
and
Ramachandran
V.N.
, Rapid Commun. Mass Spectrom , 14 , 2061 (2000 ).
22.
Yuan
H.
Mester
Z.
Lord
H.
and
Pawliszyn
J.
, J. Anal. Toxicol. 24 , 718 (2000 ).