Restricted accessResearch articleFirst published online 2003-10
Characterisation of the Intact Rainbow Trout Vitellogenin Protein and Analysis of its Derived Tryptic and Cyanogen Bromide Peptides by Matrix-Assisted Laser Desorption/Ionisation Time-of-Flight-Mass Spectrometry and Electrospray Ionisation Quadrupole/Time-of-Flight Mass Spectrometry
Vitellogenin (VTG) is a protein produced by the liver of oviparous animals. It is being used as a biomarker for exposure to endocrine disruptors in many species. Rainbow trout Vtg has recently been sequenced by the conventional cDNA nucleotide approach. We focused on protein characterization of the intact protein and its derived tryptic and cyanogen bromide peptides by matrix-assisted laser desorption/ionisation and electrospray ionisation mass spectrometry. The molecular mass of the intact protein was found to be 183127 Da. A large number of unidentified peptide ions encourage further structural analysis to propose possible sequence variants and post-translational modifications.
CaprioliR.M.SutterM., Mass Spectrometry, Introduction to Biophysical Methods for Protein and Nucleic Acid Research, Chapter 4, Ed by GlaselJ.A.DeutscherM.P., Academic Press: London, UK, p. 147 (1995).
13.
CampangniniA.FotiS.Jetschke-SchmachtelM.MaccarroneG.PrzybylskiM.SalettiR., J. Mass. Spectrom.33, 673 (1998).
KinterM.ShermanN.E., in Protein Sequencing and Identification Using Tandem Mass Spectrometry, Ed by DesiderioD.M.NibberingN.M., Wiley-Interscience (Series on Mass Spectrometry), New York, USA, p. 166 (2000).
