Abstract
A strategy for locating multiple disulfide bridges is demonstrated using two MVIIA and MVIIC ω-conotoxins in which the locations of disulfide bridges are known. The method is based on the use of selective trypsin digestion followed by non-selective pronase digestion in combination with electrospray mass spectrometry. This strategy promises to be a suitable first step in investigations applied to new peptides of unknown structure.
Get full access to this article
View all access options for this article.
References
1.
Olivera
B.M.
Rivier
J.
Scott
K.J.
Hillyard
D.R.
Cruz
L.J.
, J. Biol. Chem. 266 , 22067 (1991 ).
2.
Monje
V.D.
Haack
J.A.
Naisbitt
S.R.
Miljanich
G.
Ramachandran
J.
Nadasdi
L.
Olivera
B.M.
Hillyard
D.R.
Gray
W.R.
, Neuropharmacology 32 , 1141 (1993 ).
3.
McLntosh
M.
Cruz
L.J.
Huncapiller
M.W.
Gray
W.R.
Olivera
B.M.
Hillyard
D.R.
, Arch. Biochem. Biophys. 218 , 329 (1982 ).
4.
Nadasdi
L.
Yamashiro
D.
Chung
D.
TarczyHornoch
K.
Adriaenssens
P.
Ramachandran
J.
, Biochem. 34 , 8076 (1995 ).
5.
Woppmann
A.
Ramachandran
J.
Miljanich
P.
, Mol. Cell. Neurosci. 5 , 350 (1994 ).
6.
Chung
D.
Gaur
S.
Bell
J.R.
Ramachandran
J.
Nadasdi
L.
, Int. J. Peptide Protein Res. 46 , 320 (1995 ).
7.
Jones
A.
Bingham
J.-P.
Gerhmann
J.
Bond
T.
Loughnan
M.
Atkins
A.
Lewis
R.J.
Alewood
P.F.
, Rapid Commun. Mass Spectrom. 10 , 138 (1996 ).
8.
Caprioli
R.M.
, Mass Spectrom. Rev. 6 , 237 (1987 ).
9.
Yazdanparast
R.
Andrews
P.
Smith
D.L.
Dixon
J.E.
, Anal. Biochem. 153 , 348 (1986 ).
10.
Lange
C.
Marvin
L.
Marcual
A.
Ordsmith
N.
Bordoli
R.S.
, Rapid Commun. Mass Spectrom. 8 , 539 (1994 ).
11.
Krishnamurthy
K.
Hauer
C.R.
Prabhakaram
M.
Freedy
J.G.
Hayashi
K.
, Biol. Mass Spectrom. 23 , 719 (1994 ).
12.
Yan
L.
Tseng
J.-L.
Fridland
G.H.
Desiderio
D.M.
, J. Am. Soc. Mass Spectrom. 5 , 377 (1994 ).
13.
Prome
D.
Deon
C.
Prome
J.-C.
Wacjman
H.
Galacteros
F.
Blouquit
Y.
, J. Am. Soc. Mass Spectrom. 7 , 163 (1996 ).
14.
El Bouyoussfi
M.
Laus
G.
Verheyden
P.
Wyns
L.
Tourwe
D.
Van Binst
G.
, J. Peptide Res. 49 , 336 (1997 ).