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Abstract

The collision-dissociation behavior of two novel dimeric G-quadruplexes of HIV-1 integrase inhibitors and their non-covalent complex ions with a perylene derivative (Tel03), polyamides (ImImImβDp and PyPyPyβDp), was investigated by tandem-in-time electrospray ionization mass spectrometry (ESI-MS). It was found that the dimeric ion loses five ammonium ions one by one at activation amplitude of 10%, so the loss of NH₄⁺ is the predominant fragmentation pathway at lower collision energy. When the activation amplitude is increased to 16%, the loss of guanine nucleobases from backbones of the oligonucleotide is the predominant fragmentation pathway. And the stability of the complex ion of the dimeric G-quadruplex and Tel03 is higher than that of ImImImβDp and PyPyPyβDp. The results of the MS/MS spectra of the complex ion indicated that Tel03 binding molecules favor the stabilization of the novel G-quadruplex structure.

Keywords

tandem mass spectrometry DNA G-quadruplex non-covalent interaction HIV-1 integrase perylene polyamide

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Collision-Induced Dissociation of Dimeric G-Quadruplexes of HIV-1 Integrase Inhibitors and Their Complexes by Tandem-in-Time Mass Spectrometry

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