To establish medical use of tissue engineering technology for ligament and tendon injuries, a scaffold was developed which has sufficient ability for cell growth, cell differentiation, and mechanical properties. The scaffold made from chitosan and 0.1 per cent hyaluronic acid has adequate biodegradability and biocompatibility. An animal experiment showed that the scaffold has less toxicity and less inflammation induction. Furthermore, in-vivo animal experiments showed that the mechanical properties of the engineered ligament or tendon had the possibility to stabilize the joint. It was shown that newly developed hybrid-polymer fibre scaffold has feasibility for joint tissue engineering.
ZhuH.JiJ.LinR.GaoC.FengL.ShenJ.Surface engineering of poly (D, L-lactic acid) by entrapment of chitosan-based derivatives for the promotion of chondrogenesisJ. Biomed. Mater. Res., 2002, 62, 532–539.
12.
RoyF.DeBloisC.DoillonC. J.Extracellular matrix analogs as carriers for growth factors: in vitro fibroblast behaviorJ. Biomed. Mater. Res., 1993, 27, 389–397.
HuangL.ChengY. Y.KooP. L.LeeK. M.QinL.ChengJ. C.KumtaS. M.The effect of hyaluronan on osteoblast proliferation and differentiation in rat calvarial-derived cell culturesJ. Biomed. Mater. Res., 2003, 66, 880–884.
15.
LokeshwarV. B.IidaN.BourguignonL. Y. W.The cell adhesion molecule, GP116, is a new CD44 variant (ex14/v10) involved in hyaluronic acid binding and endothelial cell proliferationJ. Biol. Chemistry, 1996, 271, 23 853–23 864.
16.
HuangJ.HeH.ShengL.GuG.Comparison of calcium alginate film with collagen membrane for guide bone regeneration in mandibular defects in rabbitsJ. Oral Maxillofacial Surg., 2002, 60, 1449–1454.
17.
DarA.ShacharM.LeorJ.CohenS.Optimization of cardiac cell seeding and distribution in 3D porous alginate scaffoldsBiotechnol. Bioengng, 2002, 80, 305–312.
18.
DegimZ.CelebiN.SayanH.BabulA.ErdoganD.TakeG.An investigation on skin wound healing in mice with a taurine-chitosan gel formationAmino Acids, 2002, 22, 187–198.
19.
PehK.KhanT.ChingH.Mechanical, bioadhesive strength and biological evaluations of chitosan films for wound dressingJ. Pharm. Sci., 2000, 3, 303–311.
20.
TamuraH.TsurutaY.TokuraS.Preparation of chitosan-coated alginate filamentMater. Sci. Engng, 2002, C20, 143–147.
21.
MajimaT.FunakoshiT.IwasakiN.YamaneS.MinamiA.HaradaN.NonakaS.MaekawaN.TamuraY.TokuraS.MondeK.NishimauraS.Alginate and chitosan polyion complex fibers for scaffold in ligament tissue engineeringJ. Orthop. Sci., 2005, 10, 302–307.
22.
FunakoshiT.MajimaT.IwasakiN.YamaneS.MasukoT.MinamiA.HaradaK.NishimuraS.Novel chitosan-based hyaluronan hybrid polymer fibers for a scaffold in ligament tissue engineeringJ. Biomed. Mater. Res., 2005, 74, 338–346.
23.
NishimuraS.NishiN.TokuraS.Adhesion behavior of murine lymphocytes on the surface of fibrous chitin and its derivativesInt. J. Biol. Macromolecules, 1985, 7, 100–104.
24.
IwasakiN.YamaneS. T.MajimaT.KasaharaY.MinamiA.HaradaK.NonakaS.MaekawaN.TamuraH.TokuraS.ShionoM.MondeK.NishimuraS.Feasibility of polysaccharide hybrid materials for scaffolds in cartilage tissue engineering: Evaluation of chondrocyte adhesion to polyion complex fibers prepared from alginate and chitosanBiomacromolecules, 2004, 5, 828–833.
25.
FunakoshiT.MajimaT.IwasakiN.YamaneS.MasukoT.MinamiA.HaradaK.NishimuraS.Application of tissue engineering techniques for rotator cuff regeneration using a chitosan-based hyaluronan hybrid fiber scaffoldAm. J. Sports Medicine, 2005, 33, 1193–1201.
26.
MajimaT.MarchukL. L.ScioreP.ShriveN. G.FrankC. B.HartD. A.Compressive compared with tensile loading of medial collateral ligament scar in vitro uniquely influences m-RNA levels for aggrecan, collagen type II and collagenaseJ. Orthop. Res., 2000, 18, 524–531.
27.
MajimaT.LoI. K. Y.MarchukL. L.ShriveN. G.FrankC. B.Effects of ligament repair on laxity and creep behaviour of an early healing ligament scarJ. Orthop. Sci., 2006, 11, 272–277.
28.
SechriestV. F.MiaoY. J.NiyibiziC.Westerhausen-LarsonA.MatthewH. W.EvansC. H.FuF. H.SuhJ. K.GAG-augmented polysaccharide hydrogel: A novel biocompatible and biodegradable material to support chondrogenesisJ. Biomed. Mater. Res., 2000, 49, 534–541.
29.
SuhJ. K.MatthewH. W.Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: A reviewBiomaterials, 2000, 21, 2589–2598.
30.
ZimmermanE.GeigerB.AddadiL.Initial stages of cell-matrix adhesion can be mediated and modulated by cell-surface hyaluronanBiophys. J., 2002, 82, 1848–1857.
31.
HolifieldB. F.JacobsonK.Mapping trajectories of Pgp-1 membrane protein patches on surfaces of motile fibroblasts reveals a distinct boundary separating capping on the lamella and forward transport on the retracting tailJ. Cell Sci., 1991, 98, 191–203.
32.
SawaguchiN.MajimaT.IwasakiN.FunakoshiT.ShimodeK.OnoderaT.MinamiA.Extracellular matrix modulates expression of cell-surface proteoglycan genes in fibroblastsConnective Tissue Res., 2006, 47, 141–148.
33.
WooS. L.-YHollisJ. M.AdamsD. J.LyonR. M.TakaiS.Tensile properties of the human femur-anterior cruciate ligament-tibia complex. The effects of specimen age and orientationAm. J. Sports Medicine, 1991, 19, 217–225.
34.
KatoY.MukudaiY.OkimuraA.ShimazuA.NakamuraS.Effect of hyaluronic acid on the release of cartilage matrix proteoglycan and fibronectin from the cell matrix layer of chondrocyte cultures: Inter-actions between hyaluronic acid and chondroitin sulfate glycosaminoglycanJ. Rheumatology, 1995, 43 (Suppl.), 158–159.
35.
ShimazuA.JikkoA.IwamotoM.KoikeT.YanW.OkadaY.ShinmeiM.NakamuraS.KatoY.Effect of hyaluronic acid on the release of the proteoglycan from the cell matrix in rabbit chondrocyte culture in the presence and absence of cytokinesArthritis Rheum., 1993, 36, 247–253.
36.
LarsenN. E.LombadK. M.ParentE. G.BalazsE. A.Effect of hyaluroan on cartilage and chondrocyte culturesJ. Orthop. Res., 1992, 10, 23–32.
37.
YamaneS.IwasakiN.MajimaT.FunakoshiT.MasukoT.HaradaK.MinamiA.MondeK.NishimuraS.Feasibility of chitosan-based hyaluronic acid hybrid biomaterial for a novel scaffold in cartilage tissue engineeringBiomaterials, 2005, 26, 611–619.
