Why not work on T and NK cells in the Kunkel laboratory?

Abstract

Coming from Hans Wigzell’s laboratory in Uppsala, Sweden, I joined the Kunkel laboratory in 1981 to spend a post-doctoral fellowship period financed by the German Research Organization. While I initially worked with Nick Chiorazzi in an attempt to generate monoclonal antibody-secreting human B-cell hybridomas, I later studied the in vitro effects of tumor promoters on human natural killer cells and collaborated with Mary Crow on some aspects of the so-called autologous mixed lymphocyte reaction. Working in the stimulating atmosphere of the Kunkel laboratory was an exceptionalexperience.I was particularlyimpressed by the efficient organizationof clinical research at Rockefeller University. The following is a very personal resumee of my stay in the Kunkel laboratory.

Keywords

autologous mixed lymphocyte reaction chronic lymphocytic leukemia human monoclonal antibodies natural killer cells tumor promoters

Get full access to this article

View all access options for this article.

References

Kabelitz D , Tötterman TH , Gidlund M , Nilsson K , Wigzell H. Activation of human T lymphocytes by 12-O-tetradecanoylphorbol-13-acetate: role of accessory cells and interaction with lectins and allogeneic cells . Cell Immunol 1982; 70: 277-286 .

Chiorazzi N , Wasserman RL , Kunkel HG. Use of Epstein-Barr virus-transformed B cell lines for the generation of immunoglobulin-producing human B cell hybridomas . J Exp Med 1982; 156: 930-935 .

Kabelitz D , Klareskog L , Simonsson B. Density separation of chronic lymphocytic leukemia cells: low-density non-T cells are efficient stimulator cells in allogeneic and autologous mixed leukocyte reaction . Clin Immunol Immunopathol 1983; 26: 287-295 .

Crow MK , Kunkel HG. Human dendritic cells: major stimulators of the autologous and allogeneic mixed leukocyte reactions . Clin Exp Immunol 1982; 49: 338-346 .

Kabelitz D , Tötterman TH , Nilsson K , Gidlund M. Phorbol ester treated chronic B lymphocytic leukaemia cells induce autologous T cell proliferation without generation of cytotoxic T cells . Clin Exp Immunol 1984; 57: 461-466 .

Kabelitz D , Kunkel HG. Phorbolester-treated human lymphocytes are susceptible to natural killer cell-mediated cytolysis . J Immunol 1983; 130: 2505-2507 .

Kabelitz D. Modulation of natural killer susceptibility by indole alkaloid tumor promoter dihydroteleocidin B . Cell Immunol 1984; 87: 304-308 .

Kabelitz D. Modulation of natural killing by tumor promoters: the regulatory influence of adherent cells varies with the type of target cells . Immunobiol 1985; 169: 436-446 .