Detection and clinical significance of Ba2+- and Sr2+-dependent antiprothrombin antibodies in patients with systemic lupus erythematosus and antiphospholipid syndrome

Abstract

We conducted this study to confirm whether or not Ca²⁺-dependent antiprothrombinantibody (aPT) in patients’sera with systemic lupuserythematosusand=or primary antiphospholipidsyndromewould react to prothrombin=phosphatidylserinein the presenceof Sr²⁺, Mn²⁺ or Ba²⁺, or divalentmetal ions like Ca²⁺, utilizingELISA, and to analyze the clinical significance of these metal-dependentaPT. We found the presence of Ba²⁺- and Sr²⁺-dependent IgG, IgM and IgA-aPT in up to 65% of patients negative for Ca²⁺-dependent IgG-, IgM and IgA-aPT. Maximally 69% of them were complicated by antiphospholipidsyndrome-relatedsymptoms. These data suggest that the measurementof Ba²⁺- and Sr²⁺- along with Ca²⁺-dependentaPT may become a clinicallyuseful tool to correctly detect patients with antiphospholipid-related complications. However, further study is needed to clarify the clinicopathologicaldifferences among these aPTs in the future.

Keywords

antiphospholipid antibody antiprothrombin antibody lupus anticoagulant anticardiolipin antibody

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