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Abstract

We conducted this study to confirm whether or not Ca²⁺-dependent antiprothrombinantibody (aPT) in patients’sera with systemic lupuserythematosusand=or primary antiphospholipidsyndromewould react to prothrombin=phosphatidylserinein the presenceof Sr²⁺, Mn²⁺ or Ba²⁺, or divalentmetal ions like Ca²⁺, utilizingELISA, and to analyze the clinical significance of these metal-dependentaPT. We found the presence of Ba²⁺- and Sr²⁺-dependent IgG, IgM and IgA-aPT in up to 65% of patients negative for Ca²⁺-dependent IgG-, IgM and IgA-aPT. Maximally 69% of them were complicated by antiphospholipidsyndrome-relatedsymptoms. These data suggest that the measurementof Ba²⁺- and Sr²⁺- along with Ca²⁺-dependentaPT may become a clinicallyuseful tool to correctly detect patients with antiphospholipid-related complications. However, further study is needed to clarify the clinicopathologicaldifferences among these aPTs in the future.

Keywords

antiphospholipid antibody antiprothrombin antibody lupus anticoagulant anticardiolipin antibody

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References

Gromnica-Ihle E Schossler, W. Antiphospholipd syndrome . Int Arch Allergy Immunol 2000; 123: 67-76 .

Triplett PA. Protean clinical presentation of anti-phospholipidprotein antibodies (APA) . Thromb Haemostasis 1995; 74: 329-337 .

Roubey RAS. Autoantibodies to phospholipid-binding plasma proteins: a new view of lupus anticoagulants and other antiphopholipid autoantibodies . Blood 1994; 84: 2854-2867 .

Roubey RAS. Immunology of the antiphospholipid syndrome: antibodies, antigens, and autoimmune response . Thromb Haemostas 1999; 82: 656-661 .

Galli M , Comfurius P , Massen C et al. . Anticardiolipin antibodies (ACA) directed not to cardiolipin but to a plasma protein cofactor . Lancet 1990; 335: 1544-1551 .

Matsuura E , Igarashi Y , Fujimoto M et al. . Anticardiolipin cofactor(s) and differential diagnosis of autoimmune disease . Lancet 1990; 336: 177-178 .

McNeil HP , Simpson RJ , Chesterman CN , Krillis SA. Antiphospholipid antibodies are directed against a complex antigen that includes a lipid-binding inhibitor of coagulation: b2- glycoprotein I: (apolipoprotein H) . Proc Natl Acad Sci USA 1990; 87: 4120-4124 .

Roubey RAS , Eisenberg, RA , Harper MF , Winfield JB. ‘Anticardiolipin’ autoantibodies recognize b2-glycoprotein I in the absence of phospholipid. Importance of Ag density and bivalent binding . J Immunol 1995; 54: 954-960 .

Brandt JT , Triplett DA , Alving B , Scharrere I. Criteria for the diagnosis of lupus anticoagulants. An update . Thromb Haemostas 1995; 74: 1185-1190 .

10.

Greaves M , Mackie I , Machin S , Gohen H. Guidelines on the investigation and management of antiphospholipid syndrome . British Journal of. Haematology 2000; 109: 704-715 .

11.

Atsumi T , Ieko M , Bertolaccini ML et al. . Association of autoanti-bodies against the phosphoatidylserine-prothrombin complex with manifestations of the antiphospholipidsyndrome and with the presence of lupus anticoagulant . Arthritis Rheum 2000; 43: 1982-1993 .

12.

Galli M , Beretta G , Daidossi M , Bevers EM , Barbui T. Different anticoagulant and immunological properties of anti-prothrombin antibodies in patients with anti-phospholipid antibodies . Thrombo Haemostas 1997; 77: 486-491 .

13.

Galli M , Barbui T. Antiprothrombin antibodies: Detection and clinical significance in the antiphospholipid . Syndrome. Blood 1999; 93: 2149-2157 .

14.

Arvieux J , Darmige L , Caron C et al. . Development of an ELISA for autoantibodies to prothrombin showing their prevalence in patients with lupus anticoagulants . Thromb Haemostasis 1995; 74: 1120-1125 .

15.

Donohoe S , Mackie I , Isenberg D , Machin S. Anti-prothrombin antibodies: assay conditions and clinical associations in the anti-phopholipid syndrome . Br J Haematol 2000; 113: 544-549 .

16.

Borowski, M , Furie, B. C. , Bauminger, S , Furie B. Prothrombin requires two sequential metal-dependent conformational transitions to bind phospholipid . J Biol Chem 1986; 261: 14969-14975 .

17.

Hochberg MC. Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus . Arthritis Rheum 1997; 40: 1725-1725 .

18.

Wilson WA , Gharavi AE , Koike T et al. . International consensus statement on preliminary classification criteria for definite anti-phospholipid syndrome . Arthritis Rheum 1999; 42: 1309-1311 .

19.

Akimoto T , Akama T , Saitoh M , Kono I , Sumida T. Antiprothrombin autoantibodies in severe preeclampsia and abortion . Am J Med 2001; 110: 188-191 .

20.

Nelsenstuen GL , Broderius M , Martin G. Role of g-carboxyglutamic acid . J Biol Chem 1976; 251: 6886-6893 .

Detection and clinical significance of Ba2+- and Sr2+-dependent antiprothrombin antibodies in patients with systemic lupus erythematosus and antiphospholipid syndrome

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