Sage Journals: Discover world-class research

Abstract

We examined the presence of the epitope recognized by antiribosomal P protein antibody (anti-P) on the cell surface of human immunocompetent cells. Highly purified CD4/ and CD8/ T cells, and B cells from normal healthy individuals were reacted with affinity-purified IgG anti-P, and were stained with FITC-conjugated F(ab⁰)₂, fragment goat anti-human IgG, followed by analysis on flow cytometry with gating for viable cells by propidium iodide staining. The presence of an epitope that is antigenically related to the carboxyl-terminal 22-amino-acid sequence of ribosomal P protein was not demonstrated on the surface of fresh CD4/ and CD8/ T cells, or fresh B cells. However, the expression of the ribosomal P epitope was induced on CD4/ and CD8/ T cells after activation with immobilized anti-CD3, whereas the epitope was not expressed on activated B cells. These results indicate that anti-P is an antilymphocyte antibody, which reacts specifically with activated T cells but not with resting T cells or B cells, suggesting possible direct effects of anti-P on the immune dysregulation in systemic lupus erythematosus.

Keywords

human T lymphocytes lupus autoantibodies

Get full access to this article

View all access options for this article.

References

Bonfa E , Elkon KB. Clinical and serologic associations of the antiribosomal P protein antibody . Arthritis Rheum 1986; 29: 981–965 .

Elkon KB , Parnassa AP , Foster CL. Lupus autoantibodies target ribosomal P proteins . J Exp Med 1985; 162: 459–471 .

Van Agthoven AJ , Massen JP , Möller W. Structure and phosphorylation of an acidic protein from 60S ribosomes and its involvement in elongation factor-2 dependent GTP hydrolysis . Biochem Biophys Res Commun 1977; 77: 989–998 .

Elkon KB , Skelly S , Parnassa A et al. Identification and chemical synthesis of a ribosomal protein antigenic determinant in systemic lupus erythematosus . Proc Natl Acad Sci USA 1986; 83: 7419–7423 .

Bonfa E , Golombek SJ , Kaufman LD et al. Association between lupus psychosis and anti-ribosomal P protein antibodies . New Engl J Med 1987; 317: 265–271 .

Schneebaum AB , Singleton JD , West SG et al. Association of psychiatric manifestations with antibodies to ribosomal P proteins in systemic lupus erythematosus . Am J Med 1991; 90: 54–62 .

Isshi K , Hirohata S. Association of anti-ribosomal P protein antibodies with neuropsychiatric systemic lupus erythematosus . Arthritis Rheum 1996; 39: 1483–1490 .

Hulsey M , Goldstein R , Scully L , Surbeck W , Reichlin M. Anti-ribosomal P antibodies in systemic lupus erythematosus: a case control study correlating hepatic and renal disease . Clin Immunol Immunopathol 1995; 74: 252–256 .

Arnett FC , Reichlin M. Lupus hepatitis: an under recognized disease feature associated with antibodies to ribosomal P . Am J Med 1995; 99: 465–472 .

10.

Martin AL , Reichlin M. Fluctuations of antibody to ribosomal P proteins correlate with appearance and remission of nephritis in SLE . Lupus 1996; 5: 22–29 .

11.

Winfield JB. Anti-lymphocyte antibodies in systemic lupus erythematosus . Clin Rheum Dis 1985; 11: 523–549 .

12.

Winfield JB , Mimura T , Fernsten PD. Antilymphocyte autoantibodies. In: Wallace DJ , Hahn BH (eds) Dubois' Lupus Erythematosus. Lea and Febiger: Philadelphia , 1993, pp 254–259.

13.

Koren E , Reichlin MW , Koscec M , Fugate RD , Reichlin M. Auto-antibodies to the ribosomal P proteins react with a plasma membrane related target on human cells . J Clin Invest 1992; 89: 1236–1241 .

14.

Stafford HA , Chen AE , Anderson C et al. Anti-ribosomal and ‘P-peptide’-specific autoantibodies bind to T lymphocytes . Clin Exp Immunol 1997; 109: 12–19 .

15.

Tan EM , Cohen AS , Fries JF et al. The 1982 revised criteria for the classification of systemic lupus erythematosus . Arthritis Rheum 1982; 25: 1271–1277 .

16.

Amons R , Pluijms W , Möller W. The primary structure of ribosomal protein eL12=eL12-P from Artemia salina 80S ribosomes . FEBS Lett 1979; 194: 85–89 .

17.

Avrameas S. Coupling of enzymes to proteins with glutaraldehyde: use of the conjugates for the detection of antigens and antibodies . Immunochemistry 1969; 6: 43–52 .

18.

Boyum A. Isolation of mononuclear cells and granulocytes from human blood; isolation of mononuclear cells by one centrifugation, and of sedimentation of 1 g . Scand J Clin Lab Invest 1968; 21(Suppl, 97): 77–89 .

19.

Thiele DL , Kurosaka M , Lipsky PE. Phenotype of the accessory cell necessary for mitogen stimulate T and B cell responses in human peripheral blood: delineation by its sensitivity to the lysosomotropic agent, L-leucine methyl ester . J Immunol 1983; 131: 2282–2290 .

20.

Geppert TD , Lipsky PE. Accessory cell independent proliferation of human T4 cells stimulated by immobilized monoclonal antibodies to CD3 . J Immunol 1987; 138: 1660–1666 .

21.

Hirohata S , Jelinek DF , Lipsky PE. T cell-dependent activation of B cell proliferation and differentiation by immobilized monoclonal anti-bodies to CD3 . J Immunol 1988; 140: 3736–3744 .

22.

Hirohata S , Shinohara S. Bidirectional modulation of human B cell stimulation by activated T cells . Cell Immunol 1993; 150: 257–268 .

23.

Tanaka S , Matsuyama T , Steinberg AD , Schlossman SF , Morimoto C. Antilymphocyte antibodies against CD4 / 2H4 / cell population in patients with systemic lupus erythematosus . Arthritis Rheum 1989; 32: 398–405 .

24.

Mimura T , Fernsten P , Jarjour W , Winfield JB. Autoantibodies specific for different isoforms of CD45 in systemic lupus erythematosus . J Exp Med 1990; 172: 653–656 .

25.

Kumagai S , Steinberg AD , Green I. Antibodies to T cells in patients with systemic lupus erythematosus can induce antibody-dependent cell-mediated cytotoxicity against human T cells . J Clin Invest 1981; 67: 605–614 .

26.

Minota S , Winfield JB. IgG anti-lymphocyte antibodies in systemic lupus erythematosus react with surface molecules shared by peripheral T cells and a primitive T cell line . J Immunol 1987; 138: 1750–1756 .

27.

Isshi K , Hirohata S. Differential roles of the anti-ribosomal P antibody and antineuronal antibody in the pathogenesis of central nervous system involvement in systemic lupus erythematosus . Arthritis Rheum 1998; 41: 1819–1827 .

28.

Winfield JB , Shaw M , Silverman LM , Eisenberg RA , Wilson HA III , Koffler D. Intrathecal IgG synthesis and blood-brain barrier impairment in patients with systemic lupus erythematosus and central nervous system dysfunction . Am J Med 1983; 74: 837–844 .

29.

Hirohata S , Hirose S , Miyamoto T. Cerebrospinal fluid IgM, IgA, and IgG indexes in systemic lupus erythematosus: their use as estimates of central nervous system disease activity . Arch Intern Med 1985; 145: 1843–1846 .

30.

Déchanet J , Grosset C , Taupin J-L et al. CD40 ligand stimulates proinflammatory cytokine production by human endothelial cells . J Immunol 1997; 159: 5640–5647 .

Antiribosomal P protein antibody in human systemic lupus erythematosus reacts specifically with activated T cells

Abstract

Keywords

Get full access to this article

References