Suppression of experimental systemic lupus erythematosus (SLE) in mice via TNF inhibition by an anti-TNFα monoclonal antibody and by pentoxiphylline

Abstract

We have previously shown that the clinical manifestations of experimental systemic lupus erythematosus (SLE) correlate with an early increased secretion of TNFα and IL-1. In the present study, we examined the efficacy of two therapeutic modalities which lower TNFα production or activity, on the clinical manifestations of the disease. Experimental SLE was induced in naive C3H.SW mice by injection of the human anti-DNA monoclonal antibody (mAb) bearing the common idiotype, 16/6 Id. Two weeks after booster injections, treatment with either an anti-TNFα mAb, or pentoxiphylline (PTX) was started, for a period of 6 weeks. Production of TNFα (by splenocytes) and IL-1 (by peritoneal macrophages) was determined 3 and 7 months after disease induction. The experimental mice were also followed for disease manifestations. Both treatment protocols, with anti-TNFα mAb and with PTX, reduced the production of the two pro-inflammatory cytokines, TNFα and IL-1, in mice with experimental SLE. Anti-DNA antibodies were significantly lower in the mice treated with either protocol. In addition, a significantly lower rate of leukopenia, proteinuria and immune complex deposition was observed in treated mice. Abrogation of TNFα and IL-1 production in the early stages of experimental SLE by an anti-TNFα mAb or by PTX improves the clinical status of mice affiicted with this autoimmune disease.