Expression of complement regulatory proteins in diffuse proliferative glomerulonephritis

Abstract

This study assessed the expression of complement receptor 1 (CR1), decay accelerating factor (DAF) and membrane inhibitor of reactive lysis (CD59) on the erythrocytes and glomerulus of diffuse proliferative glomerulonephritis (DPGN) of systemic lupus erythematosus (SLE) patients using flow cytometry and immunofluorescence techniques to elucidate their role in the pathogenesis of DPGN. Expression of CR1 on the erythrocytes and glomerulus of DPGN patients was reduced compared with expression in normal subjects. However, expression of DAF and CD59 was increased on both erythrocytes and glomerulus of DPGN patients, suggesting the generation of a protective response against complement-mediated injury.

Keywords

complement receptor 1 (CR1)decay accelerating factor (DAF)membrane inhibitor of reactive lysis (CD59)diffuse proliferative lupus glomerulonephritis (DPGN)erythrocytes(E)

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References

Couser WG . The pathogenesis of glomerulonephritis . Kidney Int 1993; 44 (Suppl 42): S19–S20 .

Couser WG , Nangaku M , Shankland SJ , Johnson RJ . Molecular mechanisms of experimental glomerulonephritis: An overview . Nephrology 1997; 3: S633–S637 .

Nangaku M , Johnson RJ , Couser WG . Complement regulatory proteins and glomerulonephritis . Exp Nephrol 1997; 5: 345–354 .

Fischer E , Appay MD , Cook J , Kazatchkine ME . Characterization of the human glomerular C3 receptor as the C3b/C4b complement type one (CR1) receptor . J Immunol 1986; 136: 1373–1377 .

Nose M , Katoh M , Okada N , Kyogoku M , Okada H . Tissue distribution of HRF20, a novel factor preventing the membrane attack of homologous complement, and its predominant expression on endothelial cells in vivo . Immunology 1990; 70: 145–149 .

Cosio FG , Sudmak DD , Mahan JD , Nahman NS Jr . Localisation of decay accelerating factor in normal and diseased kidneys . Kidney Int 1989; 36: 100–107 .

Iida K , Nussenzweig V . Complement receptor is an inhibitor of complement cascade . J Exp Med 1981; 153: 1138–1150 .

Nicholson WA , Burge J , Fearon DT . Isolation of a human erythrocytes membrane glycoprotein with decay accelerating activity for C3 convertases of the complement system . J Immunol 1982; 129: 184–189 .

Okada N , Harada R , Fujita T , Okada H . A novel membrane glycoprotein capable of inhibiting membrane attack of homologous complement . Int Immunol 1989; 1: 205–208 .

10.

Miyakawa Y , Yamada A , Kosaka K , Tsuda F , Kosugi E , Mayumi M . Defective immune adherence (C3b) receptor on erythrocytes from patients with systemic lupus erythematosus . Lancet 1981; 2: 493–497 .

11.

Kazatchkine MD , Fearon DT , Appay MD , Mandet C , Bariety J . Immunohistochemical study of the human glomerular C3b receptor in normal kidney and in seventy five cases of renal diseases . J Clin Invest 1982; 69: 900–912 .

12.

Teixeira JE , Costa RS , Lachmann PJ , Wurzner R , Barbosa JE . CR1 stump peptide and terminal complement complexes are found in the glomeruli of lupus nephritis patients . Clin Exp Immunol 1996; 105: 497–503 .

13.

Pascual M , Steiger G , Schifferli JA . Identification of membrane bound CR1 in human urine: evidence for its release by glomerular podocytes . J Exp Med 1994; 179: 889–899 .

14.

Shibata T , Cosio FG , Birmingham DJ . Complement activation induces the expression of decay accelerating factor on human mesangial cells . J Immunol 1991; 147: 3901–3908 .

15.

Cosio FG , Shibata T , Rovin BH , Birmingham DJ . Effects of complement activation products on the synthesis of DAF and MCP by human mesangial cells . Kidney Int 1994; 46: 986–992 .

16.

Shibata T , Kohsaka T . Effects of complement activation on the expression of CD59 by human mesangial cells . J Immunol 1995; 155: 403–409 .