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Abstract

This paper describes, for the first time, how topical application in rats of a commercial preparation of lindane widely used in public health, at similar doses and routes of administration as in humans, leads to rapid absorption and accumulation of lindane in the testes. An early peak of absorption was detected in plasma 6 h after topical treatment of male Wistar rats with a commercial preparation of 1% lindane (Plomurol). Higher plasma levels were observed after repetitive doses of 60 mg/kg b.w., the amount recommended for the treatment of scabies and pediculosis in humans in several countries. A residue level of 7.4±0.67,ag/g was found in testicular tissue 6 h after a single daily topical application for 4 consecutive days. The ultrastructural study of testicular interstitial cells exposed to dermal application of lindane (Plomurol®) revealed widespread damage of a great number ofLeydig cells, some ofwhich were completely disintegrated.

Keywords

lindane rat testes Leydig cells tissue concentration plasma absorption

References

1 Nelson L. Pesticide perturbation of sperm cell function . Bull Environ Contam Toxicol 1990; 45: 876-882 .

2 Colborn T , von Saal F , Soto AM. Developmental effects of endocrine-disrupting chemicals in wildlife and humans . Health Perspect 1993; 101: 378-384 .

3 Korach KS et al. Estrogen receptor-binding activity of polychlorinated hydroxybiphenyls: conformationally restricted structural probes . Mol Pharmacol 1987; 33: 120-126 .

4 Prasad AK , Pant N , Srivastava SC , Kumar R , Srivastava SP. Effect of dermal application of hexachlorocyclohexane (HCH) on male reproductive system of rat . Hum Exp Toxicol 1995; 14: 484-488 .

5 Dalsenter PR , Faqi AS , Merker H-J , Chahoud Y. Reproductive toxicity and tissue concentrations of lindane in adult male rats . Hum Exp Toxicol 1996; 15: 406-410 .

6 Brandt I , Berg C , Halldin K , Brunstrom B. Developmental and reproductive toxicity of persistent environmental pollutants . Arch Toxicol 1998; suppl 20: 111-119 .

7 Waller DP et al. Great Lakes fish as source of maternal and fetal exposure to chlorinated hydrocarbons . Toxicol Ind Health 1996; 12: 335-345 .

8 Wiktelius S , Edwards CA. Organochlorine insecticide residues in African fauna 1972-1995 . Rev Environ Contam Toxicol 1997; 151: 1-37 .

9 Chapin RE , Harris MW , Davis BJ. The effects of perinatal/juvenile methoxychlor exposure on adult rat nervous, immune, and reproductive system function . Fundam Appi Toxicol 1997; 40: 138-157 .

10.

10 Dalsenter PR , Faqi AS , Webb J , Maerker HJ , Chahoud I. Reproductive toxicity and toxicokinetics of lindane in the male offspring of exposed during lactation . Hum Exp Toxicol 1997; 16: 146-153 .

11.

11 Ginsburg CM , Lowry W , Reisch JS. Absorption of lindane (gamma benzene hexachloride) in infants and children . J Pediatr 1977; 91: 998-1000 .

12.

12 Ronco AM , Valladares L. Phospholipid methylation decreases in human chorionic gonadotrophin-induced desensitized rat Leydig cells . J Endocrinol 1993; 138: 65-71 .

13.

13 Nigan SK et al. Serum hexachlorocyclohexane residues in workers engaged at a HCH manufacturing plant . Int Arch Occup Environ Health 1986; 57: 315-320 .

14.

14 Menczel E , Bucks D , Maibach H , Wester R. Lindane binding to sections of human skin: skin capacity and isotherm determinations . Arch Dermatol Res 1984; 276: 326-329 .

15.

15 Roy Chowdhury A , Venkatakrishna-Bhatt H , Gautam AK. Testicular changes of rats under lindane treatment . Bull Environ Contam Toxicol 1987; 38: 154-156 .

16.

16 Chowdhury AR , Gautam AK , Bhatnagar VK. Lindaneinduced changes in morphology and lipids profile of testes in rats . Biomed Biochim Acta 1990; 49: 1059-1065 .

17.

17 Suwalsky M et al. The organochlorine pesticide lindane interacts with the human erythrocyte membrane . Pestic Biochem Physiol 1998; 62: 87-95 .

Plasma absorption and ultrastructural changes of rat testicular cells induced by lindane

Abstract

Keywords

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