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Abstract

It is now possible, starting from isolated cells in culture, to regenerate higher plants, including many of importance in agriculture and horticulture. It is also possible to alter the genetic equipment of such cells and to induce the formation of fusion hybrids from them by enzymic removal of their cell walls. From such altered cells and such hybrids new plants can be generated. These technical achievements open up new possibilities in plant breeding and in the exploitation of the chemical potentialities of higher plant cells.

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References

Street

H. E.

, Excised root culture. Biol. Rev. 32, 117–55, (1957).

Butcher

D. N.

and Street

H. E.

, Excised root culture. Bot. Rev. 30, 513–586 (1964).

Street

H. E.

, Physiology of Root Growth. Annu. Rev. Plant Physiol. 17, 315–344 (1966).

Street

H. E.

, Growth in organised and unorganised systems. Knowledge gained by culture of organ and tissue explants. In Treatise of Plant Physiology, edited by Steward

F. C.

, Vol. 5B, Chapter 6, pp. 2–224. Academic Press, (1969).

Plant Tissue and Cell Culture

edited by Street

H. E.

, Bot. Monogr. 11. Blackwell Scientific Publications, Oxford and University California Press, Berkeley, (1973).

Street

H. E.

and Henshaw

G. G.

, Introduction and methods employed in plant tissue culture. In Cells and Tissues in Culture, edited by Willmer

E. N.

, Vol. 3, pp. 459–532. Academic Press, London (1966).

Yeoman

M. M.

, Tissue (Callus) Cultures—Techniques. In Plant Tissue and Cell Culture, edited by Street

H. E.

, pp. 31–58. Blackwell Scientific Publications, Oxford and University California Press, Berkeley (1973).

Cocking

E. C.

, Plant cell protoplasts—isolation and development. Annu. Rev. Plant Physiol. 23, 29–50 (1972).

Morel

and Martin

, Guérison de dahlias atteints d'une maladie à virus. C. R. Acad. Sci. 235, 1325–1325 (1952).

10.

Hollings

, Disease control through virus-free stock. Annu. Rev. Phytopathol. 3, 367–396 (1965).

11.

Walkey

, Meristem culture. In Towards plant improvement by in vitro methods, edited by Evans

P. K.

. Academic Press, London (1976).

12.

Murashige

, Plant propagation through tissue culture. Annu. Rev. Plant Physiol. 25, 135–166 (1974).

13.

Sunderland

, Nuclear cytology. In Plant Tissue and Cell Culture, edited by Street

H. E.

, pp. 161–189. Blackwell Scientific Publications, Oxford and University California Press, Berkeley (1973).

14.

Skoog

and Miller

G. O.

, Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp. Soc. Exp. Biol. II, 118–130 (1957).

15.

Street

H. E.

, Experimental embryogenesis—the totipo- tency of cultured plant cells. In The Developmental Biology of Plants and Animals, edited by Graham

C. F.

and Wareing

P. F.

, pp. 73–90. Blackwell Scientific Publications, Oxford (1976).

16.

Sunderland

, Anther culture; a progress report. Sci. Prog. 59, 527–549 (1971).

17.

Nitsch

, La culture de pollen isolé sur milieu synthétique. C. R. Acad. Sci. 278, 1031–1034 (1974).

18.

King

P. J.

and Street

H. E.

, Growth patterns in cell cultures. In Plant Tissue and Cell Culture, edited by Street

H. E.

, pp. 269–337. Blackwell Scientific Publications, Oxford and University California Press, Berkeley (1973).

19.

Street

H. E.

, Gould

A. R.

and King

, Nitrogen assimilation and protein synthesis in plant cell cultures. In Perspectives in Experimental Botany, edited by Sunderland

, Vol. 2, pp. 337–356. Academic Press, London (1975).

20.

Street

H. E.

, Plant cell cultures: Present and projected applications for studies in cell metabolism. In Genetic Manipulations with Plant Material, edited by Ledoux

, NATO Advanced Studies Institutes, Series A. Vol. 3, pp. 211–229. Plenum Publishing Co. New York (1975).

21.

King

P. J.

, Cox

B. J.

, Fowler

M. W.

and Street

H. E.

, Metabolic events in synchronised cell cultures of Acer pseudoplatanus, L. Planta, 117, 109–122 (1974).

22.

Gould

A. R.

and Street

H. E.

, Kinetic aspects of synchrony in suspension cultures of Acer pseudoplatanus L. J. Cell Sci. 17, 337–384 (1975).

23.

Street

H. E.

, Plant cell cultures: Present and projected applications for studies in genetics. In Genetic Manipulations with Plant Material, edited by Ledoux

, NATO Advanced Studies Institutes, Series A. Vol. 3, pp. 231–244. Plenum Publishing Co. New York (1975).

24.

Dix

P. J.

and Street

H. E.

, Selection of cell lines with enhanced chilling resistance. Ann. Bot. (London) 40, 903–910 (1976).

25.

Dix

P. J.

and Street

H. E.

, Sodium chloride resistant cultured cell linesfrom Nicotiana sylvestris and Capsicum annuum. Plant Sci. Lett. 5, 231–237 (1975).

26.

Marton

and Maliga

, Control of resistance in tobacco cells to 5-bromodeoxyuridine by a simple Mendelian factor. Plant Sci. Lett. 5, 77–81 (1975).

27.

Nag

K. K.

and Street

H. E.

, Freeze preservation of cultured plant cells. I. The pretreatment phase. Physiol. Plant. 34, 254–260 (1975a).

28.

Nag

K. K.

and Street

H. E.

, Freeze preservation of cultured plant cells. II. The freezing and thawing phase. Physiol. Plant. 34, 261–265 (1975b).

29.

Withers

L. A.

and Street

H. E.

, Freeze preservation of cultured plant cells. III. The pregrowth phase. Physiol. Plant. 38 (in press).

30.

Carlson

P. S.

, Smith

H. H.

and Dearing

R. D.

, Parasexual interspecific plant hybridisation. Proc. Nat. Acad. Sci. U.S.A. 69, 2292–2294 (1972).

31.

Melchers

and Labib

, Somatic hybridisation of plants by fusion of protoplasts. 1. Selection of light resistant hybrids of ‘haploid’ light-sensitive varieties of tobacco. Mol. Gen. Genet. 135, 277–294 (1974).

32.

Power

J. B.

, Frearson

E. M.

, Hayward

, George

, Evans

P. K.

, Berry

S. F.

and Cocking

E. C.

, Somatic hybridisation of Petunia hybrida and Petunia parodii. Nature (London) 263, 500–502 (1976).

33.

Kao

K. N.

, Constabel

, Michayluk

M. R.

and Gamborg

O. L.

, Plant protoplasts; fusion and growth of intergenetic hybrid cells. Planta 120, 215–227 (1974).

34.

Schieder

, Selektion einer somatishchen Hybride: nach Fusion von Protoplasten auxotrophen Mutanten von Sphaerocarpus dommellii Aust. Z. Pflanzenphysiol. 74, 357–365 (1974).

35.

Cocking

E. C.

, Power

J. P.

, Evans

P. K.

, Safwat

, Frearson

E. M.

, Hayward

, Berry

S. F.

and George

, Naturally occurring differential drug sensitivities of cultured plant protoplasts. Plant Sci. Lett. 3, 341–350 (1974).

36.

Ledoux

, Genetic manipulations with Plant Material NATO Advanced Studies Institutes Series, Series A. Vol. 3. Plenum Publishing Co. New York (1975).

37.

Braun

A. C.

and Mandle

R. J.

, Studies on the inactivation of the tumor—inducing principle in crown gall. Growth 12, 255–269 (1948).

38.

Morel

, Deviations du métabolisme azoté des tissus de crown-gall. In Les Cultures de Tissues de Plantes. Colloques Internationaux du C. N. R. S. No. 193, pp. 463–471. Paris (1971).

39.

Zaenen

, van Larebeke

, Teuchy

, van Montagu

and Schell

, Supercoiled circular DNA in crown—gall inducing Agrobacterium strains. J. Mol. Biol. 86, 109–127 (1974).

40.

Smith

H. O.

and Wilcox

K. W.

, A restriction enzyme from Hemophilus influenzae. I Purification and general properties. J. Mol. Biol. 51, 379–391 (1970).

41.

Jnr

T. J. Jelly.

and Smith

H. O.

, A restriction enzyme from Hemophilus influenzae II. Base sequence of the recognition site. J. Mol. Biol. 51, 393–409 (1970).

42.

Cassuto

, Lash

, Scriprakash

K. S.

and Radding

C. M.

, Role of exonuclease and β-protein of phage λ in genetic recombination V. Recombination of A DNA in vitro. Proc. Natl. Acad. Sci. U.S.A. 68 1639–1643 (1971).

43.

Mertz

J. E.

and Davis

, Cleavage of DNA by R, restriction endonuclease generates cohesive ends. Proc. Nat. Acad. Sci. U.S.A. 69, 3370–3374 (1972).

44.

Cohen

S. N.

, The manipulation of genes, Sci. Am. 24–33, July (1975).

Engineering with Plant Cells

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