Two collagen based nanocomposites were synthesised by initiating the reconstitution of collagen solutions in the absence or presence of alginate with simultaneous hydroxyapatite synthesis. The structural characteristics and thermal behaviours of both collagen-hydroxyapatite (M1) and collagen/alginate-hydroxyapatite (M2) were evaluated in comparison with rabbit bone (RB), which confirmed the bone-like features of synthetic nanocomposites. However, differences in microstructure, phase and composition were found among the respective samples calcined at 600 and 1000°C, as revealed by X-ray diffraction and scanning electron microscopy. Especially, in contrast to the presence of beta tricalcium phosphate in M1, pure HA was obtained in both M2 and RB after calcination at 1000°C, confirming that the introduction of alginate with Ca capturing capability is beneficial to achieve nanocomposites with better analogue to natural bone.
PalmerL. C., NewcombC. J., KaltzS. R., SpoerkeE. D. and StuppS. I.: ‘Biomimetic systems for hydroxyapatite mineralization inspired by bone and enamel’, Chem. Rev., 2008, 108, 4754–4783. doi: 10.1021/cr8004422
TadicD.: ‘A thorough physicochemical characterisation of 14 calcium phosphate-based bone substitution materials in comparison to natural bone’, Biomaterials, 2004, 25, 987–994. doi: 10.1016/S0142-9612(03)00621-5
4.
MondalB., MandalN., MondalS., MukherjeeK., MukhopadhyayS. and DeyA.: ‘Optimisation of process parameters for fabrication of nanocrystalline TiO2-hydoxyapatite based scaffold using response surface methodology’, Adv. App. Ceram., 2014, 113, 129–138. doi: 10.1179/1743676113Y.0000000126
5.
ChenZ. C., ZhangX. L., ZhouK., CaiH. and LiuC. Q.: ‘Novel fabrication of hierarchically porous hydroxyapatite scaffolds with refined porosity and suitable strength’, Adv. App. Ceram., 2015, 114, 183–187. doi: 10.1179/1743676114Y.0000000213
6.
YangF., WangJ., HouJ., GuoH. and LiuC.: ‘Bone regeneration using cell-mediated responsive degradable PEG-based scaffolds incorporating with rhBMP-2’, Biomaterials, 2013, 34, 1514–1528. doi: 10.1016/j.biomaterials.2012.10.058
7.
WangX., WangX., TanY., ZhangB., GuZ. and LiX.: ‘Synthesis and evaluation of collagen-chitosan-hydroxyapatite nanocomposites for bone grafting’, J. Biomed. Mater. Res. A, 2009, 89, 1079–1087. doi: 10.1002/jbm.a.32087
8.
WangX. M., WangX. L., MaJ. F., JiangJ. M., ZhengG. Q., ChenZ. H. and LiX. D.: ‘Versatile nanostructured processing strategy for bone grafting nanocomposites based on collagen fibrillogenesis’, Adv. App. Ceram., 2009, 108, 384–388. doi: 10.1179/174367608X372907
9.
JeeS. S., ThulaT. T. and GowerL. B.: ‘Development of bone-like composites via the polymer-induced liquid-precursor (PILP) process. Part 1: influence of polymer molecular weight’, Acta Biomater., 2010, 6, 3676–3686. doi: 10.1016/j.actbio.2010.03.036
10.
LuoD., SangL., WangX., XuS. and LiX.: ‘Low temperature, pH-triggered synthesis of collagen-chitosan-hydroxyapatite nanocomposites as potential bone grafting substitutes’, Mater. Lett., 2011, 65, 2395–2397. doi: 10.1016/j.matlet.2011.05.011
11.
NudelmanF., PieterseK., GeorgeA., BomansP. H., FriedrichH., BrylkaL. J., HilbersP. A., de WithG. and SommerdijkN. A.: ‘The role of collagen in bone apatite formation in the presence of hydroxyapatite nucleation inhibitors’, Nat. Mater., 2010, 9, 1004–1009. doi: 10.1038/nmat2875
12.
SangL., HuangJ., LuoD., ChenZ. and LiX.: ‘Bone-like nanocomposites based on self-assembled protein-based matrices with Ca2+ capturing capability’, J. Mater. Sci. Mater. Med., 2010, 21, 2561–2568. doi: 10.1007/s10856-010-4117-2
13.
LiuY., LiN., QiY. P., DaiL., BryanT. E., MaoJ., PashleyD. H. and TayF. R.: ‘Intrafibrillar collagen mineralization produced by biomimetic hierarchical nanoapatite assembly’, Adv. Mater., 2011, 23, 975–980. doi: 10.1002/adma.201003882
14.
SangL., LuoD., XuS., WangX. and LiX.: ‘Fabrication and evaluation of biomimetic scaffolds by using collagen-alginate fibrillar gels for potential tissue engineering applications’, Mater. Sci. Eng. C, 2011, C31, 262–271. doi: 10.1016/j.msec.2010.09.008
15.
SangL., WangX., ChenZ., LuJ., GuZ. and LiX.: ‘Assembly of collagen fibrillar networks in the presence of alginate’, Carbohydr. Polym., 2010, 82, 1264–1270. doi: 10.1016/j.carbpol.2010.07.005
16.
WangY., RenX., MaX., SuW., ZhangY., SunX. and LiX.: ‘Alginate-intervened hydrothermal synthesis of hydroxyapatite nanocrystals with nanopores’, Cryst. Growth Des., 2015, 15, 1949–1956. doi: 10.1021/acs.cgd.5b00113
17.
KoutsopoulosS.: ‘Synthesis and characterization of hydroxyapatite crystals: A review study on the analytical methods’, J. Biomed. Res., 2002, 62, 600–612. doi: 10.1002/jbm.10280
18.
LozanoL. F., Pena-RicoM. A., HerediaA., Ocotlan-FloresJ., Gomez-CortesA., VelazquezR., BelioI. A. and BucioL.: ‘Thermal analysis study of human bone’, J. Mater. Sci., 2003, 38, 4777–4782. doi: 10.1023/A:1027483220584
19.
SakaeT., MishimaH., KozawaY. and LeGerosR. Z.: ‘Thermal stability of mineralized and demineralized dentin: a differential scanning calorimetric study’, Connect. Tissue Res., 1995, 33, 193–196. doi: 10.3109/03008209509017001
20.
FigueiredoM., FernandoA., MartinsG., FreitasJ., JudasF. and FigueiredoH.: ‘Effect of the calcination temperature on the composition and microstructure of hydroxyapatite derived from human and animal bone’, Ceram. Int., 2010, 36, 2383–2393. doi: 10.1016/j.ceramint.2010.07.016
21.
TrebaczH. and WojtowiczK.: ‘Thermal stabilization of collagen molecules in bone tissue’, Int. J. Biol. Macromol., 2005, 37, 257–262. doi: 10.1016/j.ijbiomac.2005.04.007
22.
PereiraR., CarvalhoA., VazD. C., GilM. H., MendesA. and BartoloP.: ‘Development of novel alginate based hydrogel films for wound healing applications’, Int. J. Biol. Macromol., 2013, 52, 221–230. doi: 10.1016/j.ijbiomac.2012.09.031
23.
EtokS. E., Valsami-JonesE., WessT. J., HillerJ. C., MaxwellC. A., RogersK. D., ManningD. A. C., WhiteM. L., Lopez-CapelE., CollinsM. J., BuckleyM., PenkmanK. E. H. and WoodgateS. L.: ‘Structural and chemical changes of thermally treated bone apatite’, J. Mater. Sci., 2007, 42, 9807–9816. doi: 10.1007/s10853-007-1993-z
24.
RaynaudS., ChampionE., Bernache-AssollantD. and ThomasP.: ‘Calcium phosphate apatites with variable Ca/P atomic ratio I. Synthesis, characterisation and thermal stability of powders’, Biomaterials, 2002, 23, 1065–1072. doi: 10.1016/S0142-9612(01)00218-6
