Electrodeposition of biopolymer–glucose oxidase composites

Abstract

Composite films containing glucose oxidase in a biopolymer matrix were prepared by electrodeposition. Chitosan–glucose oxidase films were prepared by cathodic electrodeposition. Anodic electrodeposition method was developed for the deposition of alginic acid and hyaluronic acid films containing glucose oxidase. The proposed deposition mechanisms involved electrophoresis, pH changes at the electrode surfaces attributed to electrochemical reactions, coagulation of polymer macromolecules and glucose oxidase and film formation. The deposition yield was studied as a function of biopolymer and glucose oxidase concentration in the solutions. The results of the deposition yield measurements, Fourier transform infrared spectroscopy and thermogravimetric and differential thermal analyses showed the formation of composite films. Electron microscopy studies showed that the film thickness can be varied in the range of 0–3 μm by the variation in deposition time at a constant deposition voltage. The methods enabled the formation of uniform films of controlled thickness. The electrodeposition methods can be used for the fabrication of biosensors.