The purpose of the present study was to characterise the physicochemical evolution of argillite/carbon steel coupons after 10 years of interaction inside the Tournemire Underground Research Laboratory (France). Carbon steel coupons were introduced in boreholes drilled in the geological formation in contact with the argillite, and were overcored 10 years later to study the interfaces. The iron release by steel corrosion induced mineralogical dissolutions (Ca phases) and precipitations (iron oxides and hydroxides), leading to a partial clogging of the argillite porosity. A sulphur enrichment was observed in the corrosion area, linked to a potential microbial activity. The biodiversities differed depending on the steel type, indicating the influence of iron–clay interactions. This analysis has shown the presence of bacteria known for their involvement in corrosion processes and isolates able to develop at elevated temperatures.
ANDRA: ‘Dossier 2005 argile–architecture et Gestion du stockage géologique’2005. Chatenay-Malabry, France, ANDRA.
2.
CampaignoleX and Crolet JLJL: ‘Method for studying stabilization of localized corrosion on carbon steel by sulphate-reducing bacteria’Corrosion199753440–447.
3.
MagotMRavotGCampaignoleXOllivierBPatelBKCFardeauMLThomasPCroletJL and GarciaJL: ‘Dethiosulfovibrio peptidovorans gen. nov., sp. nov., a new anaerobic, slightly halophilic, thiosulfate-reducing bacterium from corroding offshore oil wells’Int. J. Syst. Bacteriol.199747818–824.
4.
LiuTLiuHHuYZhouL and ZhengB: ‘Growth of thermophile sulphate-reducing bacteria and its effect on carbon steel’Mater. Corros.200960218–224.
5.
VidelaHA: ‘Corrosion inhibition by bacteria’in ‘Manual of biocorrosion’121–135; 1996Boca Raton, FLCRC Lewis Publishers.
6.
HerreraLKVidelaHA: ‘Role of iron-reducing bacteria in corrosion and protection of carbon steel’Int. Biodeterior. Biodegrad.200963891–895.
7.
LovleyDr and PhillipsEJP: ‘Organic matter mineralization with reduction of ferric iron in anaerobic sediments’Appl. Environ. Microbiol.198651683–689.
8.
LovleyDr: ‘Dissimilatory Fe(III) and Mn(IV) reduction’Microbiol. Rev.199155259–287.
9.
LovleyDrGiovannoniSJWhiteDCChampineJEPhillipsEJPGorbyYA and GoodwinS: ‘Geobacter metallireducens gen. nov. sp. nov., a microorganism capable of coupling the complete oxidation of organic compounds to the reduction of iron and other metals’Arch. Microbiol.1993159336–344.
10.
PetrieLNorthNNDollhopfSLBalkwillDL and KostkaJE: ‘Enumeration and characterization of iron(III)-reducing microbial communities from acidic subsurface sediments contaminated with uranium (VI)’Appl. Environ. Microbiol.200369, 7467-7479.
11.
GaudinAGaboreauSTinseauEBartierDPetitSGraubyOFoctF and BeaufortD: ‘Mineralogical reactions in the Tournemire argillite after in-situ interaction with steels’Appl. Clay Sci.200943196–207.
12.
BeaucaireCMichelotJLSavoyeS and CabreraJ: ‘Groundwater characterisation and modelling of water rock equilibria in the Tournemire argillaceous formation (Aveyron, France)’Appl. Geochem.200823, 2182-2197.
13.
TremosaJArcosDMatrayJMBensenouciFGaucherECTournassatC and HadiJ: ‘Geochemical characterization and modelling of the Toarcian/Domerian porewater at the Tournemire underground research laboratory’Appl. Geochem.201227, 1417-1431.
14.
HellmuthK.-HSiitari-KauppiM and LindbergA: ‘Applications of the carbon-14-polymethylmethacrylate (PMMA) impregnation method in studies on porosity and matrix diffusion’, in ‘Scientific basis for nuclear waste management XV’, (ed. C. G. Sombret), Mater. Res. Soc. Symp. Proc.1992257649–656.
15.
KelokaskiMSiitari-KauppiMSardiniPMöriA and HellmuthK.-H: ‘Characterisation of pore space geometry by14 C-PMMA impregnation-development work for in situ studies’J. Geochem. Explor.20069045–52.
16.
UriosLMarsalFPellegriniD and MagotM: ‘Microbial diversity of the 180 million-year-old Toarcian argillite from Tournemire, France’Appl. Geochem.201227, 1442-1450.
17.
PfennigNWiddelF and TrüperHG: ‘The dissimilatory sulfate-reducing bacteria’in ‘The procaryotes’, (ed. StarrM Pet al.), Vol. 1926–940; 1981SpringerBerlin.
18.
BassoOLascourrègesJFJarryM and MagotM: ‘The effect of cleaning and disinfecting the sampling well on the microbial communities of deep subsurface water samples’Environ. Microbiol.2005713–21.
19.
UriosLAgogueHLesongeurFStackebrandtE and LebaronP: ‘Balneola vulgaris gen. nov., sp. nov., a new member of the Cytophaga–Flavobacterium–Bacteroides phylum from the NW Mediterranean Sea’Int. J. Syst. Evol. Microbiol.200656, 1883-1887.
20.
AltschulSFMaddenTLSchafferAAZhangJZhangZMillerW and LipmanDJ: ‘Gapped BLAST and PSI-BLAST: a new generation of protein database search programs’Nucl. Acids Res.199725, 3389-3402.
21.
ThompsonJDHigginsDG and GibsonTJ: ‘CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice’Nucl. Acids Res.199422, 4673-4680.
22.
SaitouM and NeiM: ‘The neighbor-joining method: a new method for reconstructing phylogenetic trees’Mol. Biol. Evol.19874406–425.
23.
FelsensteinJ: ‘Confidence limits on phylogenies: an approach using the bootstrap’Evolution198530783–791.
24.
PrakashOGihringTMDaltonDDChinKJGreenSJAkobDMWangerG and KostkaJE: ‘Geobacter daltonii sp. nov., an Fe(III)- and uranium(VI)-reducing bacterium isolated from a shallow subsurface exposed to mixed heavy metal and hydrocarbon contamination’Int. J. Syst. Evol. Microbiol.201060546–553.
25.
RamamoorthySSassHLangnerHSchumannPKroppenstedtRMSpringSOvermannJ and RosenzweigRF: ‘Desulfosporosinus lacus sp. nov., a sulfate-reducing bacterium isolated from pristine freshwater lake sediments’Int. J. Syst. Evol. Microbiol.200656, 2729-2736.
26.
Tardy-JacquenodCMagotMPatelBKMatheronR and CaumetteP: ‘Desulfotomaculum halophilum sp. nov., a halophilic sulfate-reducing bacterium isolated from oil production facilities’Int. J. Syst. Bacteriol.199848333–338.
