The Use of Multiple Methods to Validate Immunocytochemical Stains 1 2

Childs GV : Use of Immunocytochemical Techniques in Cellular Endocrinology. In Electron Microscopy in Biology. Edited by Griffith J . John Wiley & Sons, New York, 1982, p 107

Childs GV , Ellison D , Foster L , Ramaley JA : Postnatal maturation of gonadotropes in the male rat pituitary. Endocrinology 109: 1683, 1981

Childs GV , Ellison DG , Lorenzen JR , Collins TJ , Schwartz NB : Immunocytochemical studies of gonadotropin storage in developing castration cells. Endocrinology 111: 1328, 1982

Childs GV , Ellison DG , Ramaley JA : Storage of anterior lobe adrenocorticotropin in corticotropes and a subpopulation of gonadotropes during the stress-nonresponsive period in the neonatal male rat. Endocrinology 110: 1676, 1982

Childs GV , Hyde CL , Naor Z , Wahl L : EImmunocytochemical analysis of pituitary cell fractions partially purified by elutriation. Program 64th Annual Meeting, The Endocrine Society, San Francisco, 1981, p 286

Childs GV , Unabia G : Application of the avidin-biotin-peroxidase complex (ABC) method to the light microscopic localization of pituitary hormones. J Histochem Cytochem 30: 713, 1982

Childs GV , Unabia G : Application of a rapid avidin-biotin-peroxidase complex (ABC) technique to the localization of pituitary hormones at the electron microscopic level. J Histochem Cytochem 30: 1320, 1982

Coulter HD , Eide RP , Unverzagt SL : Co-localization of neurophysin- and enkephalin-like immunoreactivity in cat pituitary. Peptides 2: 51, 1981

Coulter HD , Terracio L : Preparation of biological tissue for electron microscopy by freeze-drying. Anat Rec 187 (4):477, 1977

Dacheux F : Ultrastructural localization of thyrotropin (TSH) in the porcine anterior pituitary. A comparison between pre-embedding and post-embedding methods. Cell Tissue Res 222: 299, 1982

Denef C , Hautekeete E , De Wolf A , Vanderschueren B : Pituitary basophils from immature male and female rats: distribution of gonadotrophs and thyrotrophs as studied by unit gravity sede-mentation. Endocrinology 103: 724, 1978

Dudek RW , Childs GV , Boy ne AF : Quick-freezing and freeze-drying in preparation for high quality morphology and immunocytochemistry at the ultrastructural level. J Histochem Cytochem 30: 129, 1982

Halmi NS : Immunoperoxidase staining of primate pituitaries with antibodies against the β subunits of human pituitary glycoprotein hormones. J Histochem Cytochem 29: 837, 1981

Hancock MB : DAB-nickel substrate for the differential immunoperoxidase staining of nerve fibers and fiber terminals. J Histochem Cytochem 30:578A, 1982

Hsu S-M , Raine L , Fänger H : Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29: 577, 1981

Hutson JC , Childs GV , Gardner PJ : Considerations for establishing the validity of immunocytological studies. J Histochem Cytochem 27: 1201, 1979

Hyde C , Childs G , Wahl LM , Naor Z , Catt KJ : Preparation of gonadotrope-enriched cell populations from adult rat anterior pituitary cells by centrifugal elutriation. Endocrinology 111: 1421, 1982

Larsson L-I : Immunocytochemical characterization of ACTH-like immunoreactivity in cerebral nerves and in endocrine cells of the pituitary and gastrointestinal tract by using region specific antisera. J Histochem Cytochem 28: 133, 1980

Moriarty GC : Immunocytochemistry of the pituitary glycoprotein hormones. J Histochem Cytochem 24: 846, 1976

Moriarty GC , Moriarty CM , Sternberger LA : Ultrastructural immunocytochemistry with unlabeled antibodies and the peroxidase antiperxodase complex: a technique more sensitive than radioimmunoassay. J Histochem Cytochem 21: 825, 1973

Moriarty CM , Moriarty GC : Bioactive and immunoactive ACTH in the rat pituitary: influence of stress and adrenalectomy. Endocrinology 96: 1419, 1975

Moriarty GC , Tobin RB : An immunocytochemical study of TSHΒ storage in rat thyroidectomy cells with and without D or L thyroxine treatment. J Histochem Cytochem 24: 1140, 1976

Scott AP , Lowry PJ , Ratcliffe JG , Rees LH , Landon J : Corticotrophin-like peptides in the rat pituitary. J Endocrinol 61: 555, 1974

Stoeckel ME , Dellmann HD , Porte A , Klein MJ , Stutinsky F : Corticotrophic cells in the rostral zone of the pars intermedia and in the adjacent neurohypophysis of the rat and mouse: an electron microscopic study. Z Zellforsch Mikrosk Anat 136: 97, 1973

Tougard C : Immunocytochemical identification of LH- and FSH-secreting cells at the light and electron microscopic levels. In Synthesis and Release of Adenohypophyseal Hormones. Edited by Jutisz M , McKerns KW Plenum Press, New York, 1980, p 15–39

Tougard C , Picart R , Tixier-Vidal A : Immunocytochemical localization of glycoprotein hormones in the rat anterior pituitary. A light and electron microscope study using antisera against rat subunits: a comparison between preembedding and postembedding methods. J Histochem Cytochem 28: 101, 1980

Westlund K , Childs GV : Localization of serotonin fibers in the rat adenohypophysis. Endocrinology III: in press