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Abstract

Purpose:

This study investigates two electromagnetic field (EMF) modalities on cancer cells: near-infrared pulsating light (NIR) and hyperpolarized light (HPL).

Methods:

PC3, MCF7, and HeLa were exposed to Bioptron (HPL, 40 mW/cm²) and VieLight NeuroPro (NIR, 4.59 mW/cm², 810 nm, 10 Hz) for 10–60 min, with intermittent breaks. Viability, cytoskeletal and mitochondrial morphology, ATP, and lactate levels were assessed.

Results:

NIR primarily enhanced mitochondrial ATP production, while HPL induced early morphological changes. Both modalities showed exposure dependency on viability.

Conclusion:

The analysis reveals distinct cellular responses to NIR and HPL, underscoring the importance of optimized protocols for effective EMF therapies.

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